Primary antibodies for β-actin (#12620), phospho (Thr202/Tyr204) ERK (#4370), and ERK (#9102), were from Cell Signaling Technology (Danvers, MA, USA). Antibodies for glutamic acid decarboxylase 65 (GAD65; SC-377154) and Tbr1 (SC-376258; Western blot) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for Neu-N (MAB377) and Tbr2 (AB15894) were from Millipore (Burlington, MA, USA). The antibody for Ki67 (550609) was obtained from BD Pharmingen (San José, CA, USA). Antibodies for Sox2 (ab97959), Tbr1 (ab31941; immunofluorescence), vGlut1 (ab77822) and Pax6 (ab5790) were from Abcam Inc. (Cambridge, MA, USA). Secondary fluorescent antibodies were obtained from Jackson ImmunoResearch Co. Laboratories (West Grove, PA, USA). Polyvinylidene difluoride (PVDF), membranes and molecular weight standards for Western blot were obtained from BIO-RAD (Hercules, CA, USA). The enhanced chemiluminescence (ECL) Western blotting system was from Thermo Fisher Scientific Inc. (Piscataway, NJ, USA). Zinquin, the antibody for γ amino butyric acid (GABA; A2052), and all other reagents were of the highest quality available and were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
Ab15894
Manufactured by Merck Group
Sourced in United States
The AB15894 is a precision laboratory instrument designed for accurate measurements and analytical tasks. It features advanced technology and robust construction to deliver reliable performance in a wide range of research and testing applications. The core function of this product is to provide precise and consistent data collection and analysis capabilities to support scientific investigations and industrial quality control processes.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488, by Thermo Fisher Scientific (2 mentions)
Ab77822, by Abcam (1 mentions)
Ab5790, by Abcam (1 mentions)
Ab97959, by Abcam (1 mentions)
Enhanced chemiluminescence ecl western blotting system, by Thermo Fisher Scientific (1 mentions)
Zinquin, by Merck Group (1 mentions)
Ab10543, by Abcam (1 mentions)
Mowiol 4 88, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Ab203491, by Abcam (1 mentions)
Hematoxylin, by Cell Signaling Technology (1 mentions)
Ab28486, by Abcam (1 mentions)
Enhanced chemiluminescence western blotting system, by Thermo Fisher Scientific (1 mentions)
Polyvinylidene difluoride pvdf, by Bio-Rad (1 mentions)
3 protocols using ab15894
1
Antibody Characterization for Neurodevelopment
2
Immunofluorescence Staining of Cryosections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunofluorescence was performed on 25-μm cryosections, as previously described (23 (link)). Antigen retrieval was performed in 0.01 M citrate buffer for 1 hour at 70°C. Sections were then quenched with 0.1 M glycine in 1× PBS for 30 min, followed by blocking, and primary and secondary antibody incubations in 1× PBS containing 0.2% gelatin, 150 mM NaCl, and 0.3% Triton X-100. Primary antibodies used were rat anti-histone H3 (1:500; phospho-S10, ab10543, Abcam), chicken anti-Tbr2 (1:300; ab15894, Millipore), and goat anti-EGFP (1:500; MPI-CBG). Secondary antibodies used were donkey anti-goat Alexa Fluor 488 (1:500; Thermo Fisher) and donkey anti-rat and donkey anti-chicken Cy3 (1:500; The Jackson Laboratories). Primary antibodies were incubated overnight at 4°C, and secondary antibodies, together with DAPI (1:1000; Sigma), were incubated for 2 hours at room temperature. Sections were mounted in Mowiol 4-88 (Sigma).
3
Comprehensive Antibody Panel for Oligodendrocyte Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary antibodies for β-actin (#12620), phospho-mitogenactivated protein kinase 1 and 2 (MEK1/2) (Ser217/221) (#9154), MEK1/2 (#9126), phospho-extracellular signal-regulated kinase 1 and 2 (ERK1/2) (Thr202/Tyr204) (#4370), ERK1/2 (#9102), myelin-associated glycoprotein (MAG) (#9043), myelin basic protein (MBP), #78896), and gliomaassociated oncogene homolog 1 (GLI1) (#2643) were from Cell Signaling Technology. Antibodies for 2',3'-cyclic-nucleotide 3'phosphodiesterase (CNPase) (SC-166558) and myelin oligodendrocyte glycoprotein (MOG) (SC-376138) were from Santa Cruz Biotechnology. Antibodies for oligodendrocytes transcription 2 (Olig2) (AB35128) and neuron-glial antigen 2 (NG2) (AB15894) were from Millipore. Antibodies for proteolipid protein (PLP) (ab28486) and platelet-derived growth factor receptor α (PDGFRα) (ab203491) were from Abcam Inc. The antibody for (sex determining region Y)-box 10 (Sox10) (MBS3201449) was from MyBioSource. Secondary antibodies, IHC Detection Reagents (HRP, Rabbit #8114), 3,3'-diaminobenzidine tetrahydrochloride (DAB) Substrate Kit (#8059), and hematoxylin (#14166) were obtained from Cell Signaling Technology. Polyvinylidene difluoride (PVDF), membranes, and molecular weight standards for Western blot were obtained from BIO-RAD. The enhanced chemiluminescence Western blotting system was from Thermo Fisher Scientific Inc.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!