Chicken ovalbumin (OVA) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Mouse fluorescein-conjugated antibodies against CD11c, I-Ab, CD80, CD86, CD40, and FcγRIIB were purchased from eBioscience. Enzyme-linked immunosorbent assay (ELISA) kits for the detection of mouse interleukin (IL)-12, IL-6, IL-4, and IL-13 as well as an enzyme immunoassay (EIA) kit for evaluation of prostaglandin E2 (PGE2) were bought from R&D Systems (Minneapolis, MN, USA).Rabbit anti-mouse Cox-2 and horseradish peroxidase (HRP)-conjugated anti-rabbit Ig secondary antibodies were purchased from Abcam (Cambridge, UK).
Rabbit anti mouse cox 2
Manufactured by Abcam
Sourced in United States
Rabbit anti-mouse Cox-2 is a primary antibody that recognizes the cyclooxygenase-2 (Cox-2) protein in mouse samples. Cox-2 is an enzyme involved in the production of prostaglandins and is often upregulated in response to inflammation or other cellular stresses.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit anti mouse cox 2
1
Immunophenotyping of Dendritic Cells
2
Measuring Neuroinflammation after BBB Disruption
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The neuronal inflammatory responses following the disruption of BBB were measured with the immunodetection of GFAP and COX-2 as described previously [4 (link),6 (link)]. The 20 μm cryosections were blocked with 5% goat serum for 30 min. Either of rabbit anti-mouse GFAP (1:200, Abcam) or rabbit anti-mouse COX-2 (1:200, Abcam) antibody was added onto the sections, and incubated for 20 h at 4˚C. Goat anti-rabbit IgG conjugated with Alexa488 (1:200) was then applied to the sections for 2 h at 22˚C. Semi-quantitative parenchymal abundance of GFAP and COX-2 were determined within the HPF and CTX as described for IgG and apo B.
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