HCC cell lines (5 x 103 / well) were seeded onto 96-well plates and, cultured for one day. Cells were incubated with different doses of Fuc-Lip-sorafenib after treatment with or without SAHA for 6 h. After incubation for 2 h, cells were washed twice with PBS, and then resuspended in medium containing serum and antibiotics. After 48 h culture, BrdU cell proliferation assay reagent (Cell Signaling) was added, and the proliferation assay was performed according to the manufacturer’s instructions. Viable cells were expressed as percentage of control.
Brdu cell proliferation assay reagent
Manufactured by Cell Signaling Technology
Sourced in United States
The BrdU cell proliferation assay reagent is a laboratory tool used to measure cell proliferation. It contains bromodeoxyuridine (BrdU), a synthetic nucleoside that is incorporated into the DNA of dividing cells. This allows for the quantification of cell proliferation through various detection methods.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using brdu cell proliferation assay reagent
1
Fuc-Lip-sorafenib Inhibits HCC Proliferation
2
Evaluating Myeloma Cell Proliferation and Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The inhibitory effect of DFX on MM cell line growth was assessed by WST-1 assay, as described previously [42 (link)]. To analyze the proliferation of MM cells with or without BMSCs, the rate of DNA synthesis was determined by BrdU assay (BrdU cell proliferation assay reagent, Cell Signaling Technology, Danvers, MA). Apoptosis was evaluated by PARP immunoblotting and quantified by using an Annexin V/7-AAD staining kit in accordance with the manufacturer's instructions (Annexin V PE Apoptosis Detection Kit I, BD Biosciences, San Jose, CA), followed by analysis on a BD FACS Canto II instrument using FACSDiva (BD Biosciences) [43 (link)].
3
Fuc-Liposome-daunorubicin's Effect on Leukemia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human leukemia cell lines (2 × 104 cells/well) were seeded into 24-well plates, and cultured for one day in medium supplemented with 10% fetal bovine serum, 5% L-glutamine, and 1% antibiotics. Cells were then incubated with different doses of Fuc-Liposome-daunorubicin. After 2 h incubation, cells were washed twice with PBS and finally resuspended in medium containing serum and antibiotics. After culture for 72 h, a BrdU cell proliferation assay reagent (Cell Signaling, Boston, MA, USA) was added and the assay performed according to manufacturer's instructions. Experiments were performed in triplicate and repeated at least twice.
4
Evaluating Myeloma Cell Growth and Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MM cells (5 x103) were treated for 48 h with CP at the indicated concentrations in 96-well plates. Subsequently, the inhibitory effect of CP on MM cell line growth was assessed using a WST-1 assay (Premix WST-1 Cell Proliferation Assay, Takara Bio, Otsu, Japan) and an Infinite M1000 PRO microplate reader (Tecan Japan, Kawasaki, Japan) as described previously [34 (link)]. The half-maximal inhibitory concentration (IC50) was defined as the drug concentration resulting in 50% cell survival relative to that of DMSO-treated cells. To analyze the proliferation of MM cells with or without BMSCs, we used a BrdU assay (BrdU cell proliferation assay reagent, Cell Signaling Technology, Danvers, MA) [5 (link)]. Initially, this assay was established using BrdU incorporated into cellular DNA during cell proliferation, and it is similar to the 3H-incorporation assay but does not use radioactive reagents. Therefore, the BrdU assay is suitable for this proliferation assay.
Apoptosis was evaluated by PARP, caspase-3, caspase-8, and caspase-9 western blotting and quantified using an Annexin V/7-AAD staining kit (BD Biosciences, San Jose, CA), as per the manufacturer’s instructions, followed by analysis on a BD FACS Canto II using FACSDiva (BD Biosciences, Tokyo, Japan) [35 ].
Apoptosis was evaluated by PARP, caspase-3, caspase-8, and caspase-9 western blotting and quantified using an Annexin V/7-AAD staining kit (BD Biosciences, San Jose, CA), as per the manufacturer’s instructions, followed by analysis on a BD FACS Canto II using FACSDiva (BD Biosciences, Tokyo, Japan) [35 ].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!