Si agap2 as1

The silencing RNA against AGAP2-AS1 (si-AGAP2-AS1) and hnRNPA2B1 (si-hnRNPA2B1) were purchased from RiboBio (Guangzhou, China). Negative control siRNA was purchased from Invitrogen (CAT#12935-110, Shanghai, China). Green fluorescence protein (GFP) was used to show transfection efficiency. Cell lines were transfected using Lipofectamine 2000 (Life Technologies, USA) at the concentration of 100 nM, according to the manufacturer’s instructions. The sequences of small interfering RNAs were: si-AGAP2-AS1#1 5′-CCACTCCACCTCAAACTCTTACCTT-3′; si-AGAP2-AS1#2 5′-GGGTCATTAAGGGACAGAGTTCAAG-3′; si-AGAP2-AS1#3 5′-CAGGTGGACTCACAATTCCAAATAT-3′; si-hnRNPA2B1 5′-GCGGAAUUAAAGAAGAUACTT-3′.

In short, the oligonucleotides (RiboBio, Guangzhou, China): si-AGAP2-AS1: 5′-AUAAAGCAGGUAACAAGUGGG-3’ (sense), 5′-CACUUGUUACCUGCUUUAUAA-3′(antisense); si-TGF-β1: 5′-ACGGAAAUAACCUAGAUGGGC-3’ (sense), 5′-CCAUCUAGGUUAUUUCCGUGG-3′(antisense), miR-486-3p mimic/inhibitor (miR-486-3p/miR-486-3p in) and their controls (si-NC, miR-NC, and miR-NC in), and plasmids (GenePharma, Shanghai, China): pcDNA and pcDNA-TGF-β1 (TGF-β1, NM_000660.7), were collected in this research. Using Lipofectamine 3000 (Invitrogen, #L3000075), 50 nM oligonucleotides and 6 μg plasmids were transfected into GBM cells at 70 % confluence for 48 h, followed by the assessment of the transfection efficiency.