Single-cell suspensions of E1KD, L1P and L2P cells were incubated in PBS/1% BSA containing PE-conjugated CD24 (M1/69) and FITC-conjugated CD29 (HMβ1-1) antibody (Biolegend) for 30 min on ice, as per manufacturer’s instructions. Cells were then washed three times in PBS/1% BSA and resuspended in 500 µl PBS. Samples were analyzed using the CyAn ADP Cellular Analyzer and Summit software (Beckman Coulter).
Pe conjugated cd24 m1 69
Manufactured by BioLegend
PE-conjugated CD24 (M1/69) is a fluorochrome-labeled antibody that binds to the CD24 antigen. CD24 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein expressed on various cell types. The PE (phycoerythrin) fluorochrome is conjugated to the antibody, allowing for detection and analysis of CD24-expressing cells using flow cytometry.
Automatically generated - may contain errors
2 protocols using pe conjugated cd24 m1 69
1
Cell Surface Marker Analysis
2
Cell Surface Marker Analysis
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Single-cell suspensions of E1KD, L1P and L2P cells were incubated in PBS/1% BSA containing PE-conjugated CD24 (M1/69) and FITC-conjugated CD29 (HMβ1-1) antibody (Biolegend) for 30 min on ice, as per manufacturer’s instructions. Cells were then washed three times in PBS/1% BSA and resuspended in 500 µl PBS. Samples were analyzed using the CyAn ADP Cellular Analyzer and Summit software (Beckman Coulter).
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