Genesys uv vis spectrophotometer

UV-VIS spectroscopy was performed by a Genesys UV-VIS spectrophotometer (Thermo Scientific, Waltham, MA, USA). The absorption spectrum was recorded from 340 nm to 700 nm. The samples were measured during different time intervals between 0 and 6 h and after 24 h of incubation. The mixtures were stored at room temperature in quartz cuvettes and in day–night light conditions in between measurements. Also, blanks (without AgNPs) of an IDL and an LB medium mixed in a 1:1 ratio with the solvent specific for the stabilization type of the AgNPs were measured. Moreover, a visual analysis of the samples was performed in parallel.

Total genomic DNA was isolated from lizard blood by using the standard phenol-chloroform extraction method with proteinase K and resuspended in TE buffer, pH 8.0. The samples were kept at +4 °C until they were processed. The DNA degradation and contamination were checked through agarose gel electrophoresis on 0.8% agarose gel followed by quantitation at 260 nm using Genesys® UV-Vis spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Afterwards, three DNA samples were shipped to Macrogen (Seoul, Korea) for genomic library preparation and sequencing. The 10x Chromium library was prepared according to the manufacturer’s instructions and sequenced on a HiSeq 2500 with a pair-end 150 bp reads.