Mammalian mitochondria isolation kit

Manufactured by Abcam

Sourced in United States

The Mammalian Mitochondria Isolation Kit is a laboratory tool designed to isolate mitochondria from mammalian cells. It provides a reliable and efficient method to extract this organelle, which plays a crucial role in cellular energy production and other biological processes.

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Lab products found in correlation

Picoprobe acetyl coa fluorometric assay kit, by Abcam (1 mentions) Nuclear cytosol fractionation kit, by Abcam (1 mentions)

4 protocols using mammalian mitochondria isolation kit

Quantifying Subcellular Acetyl-CoA Levels

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Nucleocytosolic fractions were prepared using Nuclear/Cytosol Fractionation Kit (Biovision) and Mammalian Mitochondria Isolation Kit (Biovision), following the manufacturer’s instructions. Fractions were deproteinized following perchloric acid/KOH protocol. Acetyl-CoA in nuclear-cytoplasmic fractions were quantified using PicoProbe™ Acetyl CoA Fluorometric Assay Kit (Biovision) as per manufacturer’s instructions.

Kumari R., Deshmukh R.S, & Das S. (2019). Caspase-10 inhibits ATP-citrate lyase-mediated metabolic and epigenetic reprogramming to suppress tumorigenesis. Nature Communications, 10, 4255.

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Mitochondrial Isolation from Mammalian Cells

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Mitochondria was isolated by using Mammalian Mitochondria Isolation Kit (Biovision), following the manufacture’s instruction.

Qiu X., Jiang S., Xiao Y., He Y., Ren T., Jiang L., Liu R, & Chen Q. (2021). SOX2-dependent expression of dihydroorotate dehydrogenase regulates oral squamous cell carcinoma cell proliferation. International Journal of Oral Science, 13, 3.

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Isolation of Cardiac Mitochondria

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Freshly isolated mitochondria were prepared from the hearts or from cultured cardiomyocytes using a Mammalian Mitochondria Isolation Kit (Biovision, Exton, PA, USA) according to the protocols provided by the manufacturer.

Su Q., Xu Y., Cai R., Dai R., Yang X., Liu Y, & Kong B. (2021). miR-146a inhibits mitochondrial dysfunction and myocardial infarction by targeting cyclophilin D. Molecular Therapy. Nucleic Acids, 23, 1258-1271.

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Brucella Infection of RAW 264.7 Cells

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RAW 264.7 cells were seeded in 6-well plates 24 h before Brucella infection (150,000 cells/well). At 48 h PI, cells were washed thrice with PBS and mitochondria-enriched fractions were prepared using the mammalian mitochondria isolation kit for tissue and cultured cells, according to the manufacturer’s protocol (BioVision, Milpitas, USA). These fractions were processed as described in the following section.

Lobet E., Willemart K., Ninane N., Demazy C., Sedzicki J., Lelubre C., De Bolle X., Renard P., Raes M., Dehio C., Letesson J.J, & Arnould T. (2018). Mitochondrial fragmentation affects neither the sensitivity to TNFα-induced apoptosis of Brucella-infected cells nor the intracellular replication of the bacteria. Scientific Reports, 8, 5173.

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