To further assess their viability, the cells were incubated with 50 mM calcein acetoxymethyl ester (Calcein-AM, Molecular Probes, Eugene, OR, USA) for 20 min at 37 ◦C and stained cells were then visualized under a fluorescence microscope (OLYMPUS IX71, Tokyo, Japan) at 20× magnification. Digital images were acquired with a charge-coupled device (CCD) camera (Axiocam b/w, Zeiss).
Axiocam b w
Manufactured by Zeiss
Sourced in Germany
The Axiocam b/w is a high-performance digital camera designed for microscopy applications. It features a monochrome sensor that provides high-resolution, high-sensitivity imaging. The camera is compatible with a wide range of Zeiss microscopes and can be used for various imaging tasks, such as brightfield, phase contrast, and fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using axiocam b w
1
Cell Viability Assessment by Calcein-AM
2
Visualizing Autophagy in Arabidopsis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GFP-ATG8e transgenic seedlings were observed and photographed using a Zeiss Axio Imager.A2 upright microscope (Zeiss, Oberkochen, Germany) equipped with Zeiss Axiocam BW/color digital cameras and a GFP-specific filter at the Iowa State University Microscopy and Nanoimaging Facility. Cells within the root elongation zone were photographed and the number of autophagosomes in each image was counted and averaged from at least 10 images per sample [87 (link)]. For monodansylcadaverine (MDC) staining, Arabidopsis seedling roots were stained with MDC (Sigma-Aldrich, 30432) as described previously [88 (link)]. MDC-stained seedlings were observed and imaged with the same fluorescence microscopy system using a DAPI-specific filter.
3
GFP-ATG8e Imaging and Autophagosome Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GFP-ATG8e transgenic seedlings were observed and photographed using a Zeiss Axio Imager.A2 upright microscope (Zeiss, Oberkochen, Germany) equipped with Zeiss Axiocam BW/color digital cameras and a GFP-specific filter at the Iowa State University Microscopy and Nanoimaging Facility.
Cells within the root elongation zone were photographed and the number of autophagosomes in each image was counted and averaged from at least 10 images per sample 83 . For monodansylcadaverine (MDC) staining, Arabidopsis seedling roots were stained with MDC (Sigma-Aldrich, 30432) as described previously 63 . MDC-stained seedlings were observed and imaged with the same fluorescence microscopy system using a DAPI-specific filter.
Cells within the root elongation zone were photographed and the number of autophagosomes in each image was counted and averaged from at least 10 images per sample 83 . For monodansylcadaverine (MDC) staining, Arabidopsis seedling roots were stained with MDC (Sigma-Aldrich, 30432) as described previously 63 . MDC-stained seedlings were observed and imaged with the same fluorescence microscopy system using a DAPI-specific filter.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!