Surface-sterilized Col-0 seeds were resuspended in 0,1% agarose and transferred to solid ½ MS medium supplemented with sterile filtered 4.6 ug/mL α-amanitin (Sigma-Aldrich, CAS 23109-05-9) and 9 ug/mL zebularine (Sigma-Aldrich; CAS 3690-10-6). For flagellin treatment, 2-week-old plants grown on ½ MS media supplemented with α-amanitin and zebularine were transferred to the equal ZA medium but also supplemented with 0.5 ug/mL flagellin (Flagellin from Salmonella typhimurium, Sigma-Aldrich, SRP8029-10UG). For ABA treatment, 2-week-old plants grown on ½ MS media supplemented with α-amanitin and zebularine were transferred to the equal ZA medium but also supplemented with abscisic acid at final concentrations of 100 uM. For heat-stress treatment, 2-week-old plants grown on ½ MS media supplemented with α-amanitin and zebularine were subjected to elevated temperature (4 °C for 24 h followed by 37 °C for 24 h, HS). For mild heat stress treatment 2 weeks-old Col-0 ddm1 plants were subjected to 30 °C for 24 h.
Flagellin from salmonella typhimurium
Manufactured by Merck Group
Flagellin from Salmonella typhimurium is a bacterial protein that forms the filament of the flagellum. It is a commonly used component in research and development applications involving cell signaling, immune response, and bacterial motility studies.
Automatically generated - may contain errors
Lab products found in correlation
α amanitin, by Merck Group (1 mentions)
Zebularine, by Merck Group (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Zymosan, by Merck Group (1 mentions)
L6529, by Merck Group (1 mentions)
Atglistatin, by Merck Group (1 mentions)
Ns 398, by Merck Group (1 mentions)
Poly 1 c, by Merck Group (1 mentions)
Zymosan from saccharomyces cerevisiae, by Merck Group (1 mentions)
2 protocols using flagellin from salmonella typhimurium
1
Transcriptional Regulation Under Stress
2
Activated Macrophage Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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BMDMs were treated with LPS from E. coli (O55:B5; L6529; Sigma-Aldrich) and flagellin from Salmonella typhimurium (Sigma-Aldrich) at a concentration of 100 ng/mL, Pam3CysK4 (L2000; EMC Microcollections) at a concentration of 5 µg/mL, poly:IC (Sigma-Aldrich) at a concentration of 20 µg/mL, and Zymosan from Saccharomyces cerevisiae (Sigma-Aldrich) at a concentration of 100 µg/mL. Zymosan was opsonized to increase uptake by phagocytosis and sonicated just before use. Atglistatin (SML1075; Sigma-Aldrich) was used at a concentration of 20 μM, and cells were pretreated for 1 h before further treatment. C75 (C5490; Sigma-Aldrich) was used at a concentration of 5 µg/mL, and cells were pretreated for 1 h before further treatment. Indomethacin (I7378; Sigma-Aldrich) was used at 10 µM and NS-398 (N194; Sigma-Aldrich) was used at 1 µM, and cells were pretreated for 1 h before further treatment. PGE2 (2296; Tocris, Bio-Techne) was added at 0.1 µM 1 h after treatment with LPS.
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