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Kir2dl1

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KIR2DL1 is a type I transmembrane glycoprotein that functions as an inhibitory receptor on natural killer (NK) cells. It recognizes and binds to specific major histocompatibility complex (MHC) class I molecules, providing an inhibitory signal that regulates NK cell activation and cytotoxicity.

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Lab products found in correlation

Kir2dl3, by R&D Systems (2 mentions) Ki 67, by Miltenyi Biotec (1 mentions) Nkg2c, by R&D Systems (1 mentions) Kir2dl1 s1, by Beckman Coulter (1 mentions) Nkg2c, by Miltenyi Biotec (1 mentions) Bcl 2, by BioLegend (1 mentions) Cd122, by BD (1 mentions) Lag 3, by Miltenyi Biotec (1 mentions) Igg2a, by BioLegend (1 mentions) Nkp30, by R&D Systems (1 mentions) Nkp44, by R&D Systems (1 mentions) F ab 2 goat anti human igg pe secondary antibody, by Thermo Fisher Scientific (1 mentions) Bio plex 200, by Bio-Rad (1 mentions) Lag 3, by R&D Systems (1 mentions)

Spelling variants of this product

Anti-Human KIR2DL1/CD158a FITC (3 citations) KIR2DL1-Fc (3 citations) Anti-KIR2DL1-FITC (3 citations) KIR2DL1-fluorescein isothiocyanate (2 citations) 143211-FITC KIR2DL1-specific antibody (2 citations)

2 protocols using kir2dl1

1

Comprehensive Immunophenotyping Protocol

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The following antibodies were used for flow cytometry: BD Bioscience: CD3 epsilon (UCHT1, SP34–2 and SK7), CD122 (Mik-β3), CD8a (RPA-T8), PLZF (RI7–809). CD107a (H4A3), IFN-γ (B27), KIR3DL1 (DX9), KIR2DL2/L3/S2 (CH-L), CD16 (3G8), CD57 (NK-1), HLA-DR (G46–6), HLA-C (DT9), and CD86 (FUN-1). Biolegend: CD2 (RPA-2.10), PD-1 (EH12.2H7), Bcl-2 (100), CD3 epsilon (SK7, OKT3, HIT3a and APA1/1), HLA-ABC (W6/32), NKp46 (9E2), IgG1 (MOPC-21), IgG2a (MOPC-173), CD5 (UCHT2), Lck (LCK-01), and Bcl11b (25E6). Beckman Coulter: CD56 (NKH-1), KIR2DL2/L3/S2 (GL183), KIR2DL1/S1 (EB6), NKp46 (BAB281), CD247 (TIA-2), CD69 (TP1.55.3). Miltenyi: NKG2A (REA110), NKG2C (REA205), PD-1(PD1.3.1.3), LAG-3 (REA351), CD58(TS2/9), Ki-67 (REA183), Bw4 (REA274), and TCRrd (11F2). R&D Systems: NKG2C (134591), KIR2DL1 (143211), and KIR2DL3 (180701). Millipore: FcεRI (FCABS400F), HCMV-IEA (8B1.2). ThemoFisher: LIR-1 (HP-F1), TCRab (WT13), and CD8b (SIDI8BEE). Abcam: CD3 gamma (EPR4517), CD3 delta (EP4426) and Bcl11b (25E6). Jackson Immunoresearch: Donkey anti-rabbit IgG (H+L). The following antibodies were used for western blot: Biolegend: CD3 epsilon (SK7), IgG1 (MOPC-21), β-actin (W16197A), CD247 (6B10.2), pLck (A18002D), Lck (LCK-01), CD2 (RPA-2.10), and CD16 (3G8); pCD247 (C415.9A, Santa Cruz Biotechnology), CD3 gamma (EPR4517, Abcam), and CD3 delta (EP4426, Abcam).
Wu Z., Lau C.M., Sottile R., Le Luduec J.B., Panjwani M.K., Conaty P.M., Srpan K., Laib Sampaio K., Mertens T., Adler S.P., Hill A.B., Barker J.N., Cheung N.K., Sun J.C, & Hsu K.C. (2021). Human Cytomegalovirus Infection Promotes Expansion of a Functionally Superior Cytoplasmic CD3+ NK Cell Subset with a Bcl11b-Regulated T Cell Signature. The Journal of Immunology Author Choice, 207(10), 2534-2544.
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2

Screening of HLA Class II Ligands

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Screening of HLA class II-coated beads was performed using the LABScreen Single Antigen HLA class II—Group 1 kit (OneLambda). Recombinant human Fc constructs (LAG-3, NKp30, NKp44, NKp46, KIR2DL3, KIR2DL1, KIR2DS1, KIR3DS1, KIR3DL1, KIR3DL2 and KIR2DL4) were purchased from R&D Systems, diluted in PBS to concentrations ranging from 1 to 100 µg ml−1 and incubated with a mixture of 95 HLA class II-coated beads for 30 min at room temperature. Samples were washed and incubated with F(ab′)2 goat-anti-human IgG PE secondary antibody (Life Technologies) for 30 min at 4 °C. Fc construct binding to HLA class II-coated beads was quantified using Luminex xMAP technology on a Bio-Plex 200 (Bio-Rad Laboratories).
Niehrs A., Garcia-Beltran W.F., Norman P.J., Watson G.M., Hölzemer A., Chapel A., Richert L., Pommerening-Röser A., Körner C., Ozawa M., Martrus G., Rossjohn J., Lee J.H., Berry R., Carrington M, & Altfeld M. (2019). A subset of HLA-DP molecules serve as ligands for the natural cytotoxicity receptor NKp44. Nature immunology, 20(9), 1129-1137.
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