Curare

Manufactured by Merck Group

Sourced in United States

Curare is a laboratory equipment product manufactured by Merck Group. It is a device used for the precise measurement and delivery of small volumes of liquids or gases in a controlled and consistent manner. The core function of Curare is to facilitate accurate and reproducible experimental procedures in various scientific and research applications.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using curare

Calcium Oscillations Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Calcium activity was visualized with Fluo-8 AM (Abcam, ab112129) following the manufacturer’s protocol. Briefly, cells were washed twice with PBS(−), and incubated with a mixture of Component A, B, and C at 37 °C for 30 min with or without 10 μM Tubocurarine Chloride Pentahydrate (Curare, Sigma, T2379). After 2 washes with the muscle medium_2, the Fluo-8 signals were recorded in the absence or presence of Curare using the Thunder Imager Live Cell & 3D assay (Leica, LAS X software) every 100 msec.
For analyses, the Fluo-8 intensity from a randomly selected cell was measured using ImageJ. The signal was denoised using the Savitzky-Golay filter with a 50 window size using Python. The amplitudes between a signal peak and its adjacent two valleys were measured, and the peak was counted only if the smaller amplitude was larger than half of the larger amplitude. The total number of oscillation peaks in 2 min was manually counted.

Sanaki-Matsumiya M., Villava C., Rappez L., Haase K., Wu J, & Ebisuya M. (2024). Self-organization of vascularized skeletal muscle from bovine embryonic stem cells. bioRxiv.

+ Open protocol

+ Expand

Zebrafish Embryo Adrenergic and Cholinergic Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols

Zebrafish embryos were manually arrayed into 96‐well‐plates as described above. We conducted preliminary dose response for Curare (1, 3, 5, 10 mM) and Alfuzosin (1, 3, 5, 10 mM). Concentrations of 3 mM Alfuzosin and 5 mM Curare resulted in strong effects and limited toxicity and were selected for further experiments. Zebrafish embryos were pretreated with adrenergic blocker (Alfuzosin, Prestwick Chemical Library) or cholinergic blocker (Curare, Sigma). After 2 h of treatment, adrenergic or cholinergic Hits identified in our screen (Oxymetazoline Hydrochloride, Xylometazoline Hydrochloride, Aceclidine Hydrochloride, and Tropicamide) were respectively added to the fish water at a 10 μM concentration. Plates were incubated in an automated incubator until 48 hpf, when drugs were automatically washed five times with E3 medium. Embryos from each well were then analyzed at using Zebrabox at 5 dpf as described in the “Miniaturized locomotion assay” section.

Lescouzères L., Hassen‐Khodja C., Baudot A., Bordignon B, & Bomont P. (2023). A multilevel screening pipeline in zebrafish identifies therapeutic drugs for GAN. EMBO Molecular Medicine, 15(7), e16267.

+ Open protocol

+ Expand

Detailed Neuromuscular Receptor Pharmacology

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following reagents were used at the following concentrations: P2Y₁R agonists ATP and ADP (Sigma; 10 or 20 μM); P2Y₁R antagonist MRS2500 (Tocris; 1 μm); P1R agonist adenosine (Sigma; 100 μM); pan-P2 antagonist suramin (Sigma; 100 μM); pan-muscarinic agonist muscarine (Sigma; 10 μM); pan-muscarinic blocker atropine (Sigma; 10 μM); pan-nicotinic agonist nicotine (Sigma; 50 μM); pan-nicotinic antagonist curare (Sigma; 200 μM); pan-cholinesterase inhibitor neostigmine (Sigma; 1 μM); sarco-/endoplasmic reticulum Ca^2+-ATPase (SERCA) inhibitor, cyclopiazonic acid (Sigma; CPA; 10 μM); potassium chloride (Sigma; 2–10 mM); GIIIb μ-conotoxin (Peptides International; 2.3 μM); skeletal muscle myosin-blocker 3-(N-butylethanimidoyl)−4-hydroxy-2H-chromen-2-one (BHC; Hit2lead; 100 μM); 488-, 594-, 633-conjugated-α-bungarotoxin (α-BTX; Biotium; 1 μg/mL).

Heredia D.J., Feng C.Y., Hennig G.W., Renden R.B, & Gould T.W. (2018). Activity-induced Ca2+ signaling in perisynaptic Schwann cells of the early postnatal mouse is mediated by P2Y1 receptors and regulates muscle fatigue. eLife, 7, e30839.

+ Open protocol

+ Expand

Immobilized Frog AEP Recording Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animals were immobilized with an injection of d-tubocurare (Sigma-Aldrich; St. Louis, MO, USA) into the dorsal lymph sac (3 μg/kg X. amieti; 8 μg/kg X. petersii; 10 μg/kg X. borealis, X. laevis). Frogs were maintained at ambient temperature (22.5 – 24 °C) and were draped with a wet Kimwipe (Kimberly-Clark Global Sales, LLC; Roswell, GA, USA) to maintain skin moisture. Oxygen levels were maintained via cutaneous respiration and air lung reserves. Animals were pre-treated with subcutaneous lidocaine prior to AEP electrode insertion. If an animal regained motility during recording, supplemental curare (3 μg/kg; Sigma-Aldrich; St. Louis, MO, USA) was administered via the dorsal lymph sac followed by a 15 – 30 minute pause in recording. After recording, animals were allowed to recover on a float in their home tank, while draped in a wet Kimwipe to maintain skin moisture.

Hall I.C., Woolley S.M, & Kelley D.B. (2015). Sex differences and endocrine regulation of auditory-evoked, neural responses in African clawed frogs (Xenopus). Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology, 202(1), 17-34.

+ Open protocol

+ Expand

Formation of Neuromuscular Junctions

Check if the same lab product or an alternative is used in the 5 most similar protocols

C2C12 myoblasts (ATCC) were expanded in DMEM with 10% FBS and penicillin/streptomycin. When the culture reached 70% confluency, the medium was switched to 2% horse serum containing medium to induce multinucleated myotubes. iMNs were added to the myotubes in motor neuron medium to induce formation of neuromuscular junctions and spontaneous contractions. After 3–4 weeks, the myotube contractions were observed under the microscope and were inhibited by adding 100 μM curare (Sigma). Neuromuscular junctions were observed by labeling with α-bungarotoxin conjugated with Alexa 555 (Invitrogen, 1:200) and immunostaining with myosin heavy chain (MHC, DSHB), acetylcholine receptor (AChR, DSHB), and synaptic vesicle 2 (SV2, DSHB).

Lee H., Lee H.Y., Lee B.E., Gerovska D., Park S.Y., Zaehres H., Araúzo-Bravo M.J., Kim J.I., Ha Y., Schöler H.R, & Kim J.B. (2020). Sequentially induced motor neurons from human fibroblasts facilitate locomotor recovery in a rodent spinal cord injury model. eLife, 9, e52069.

+ Open protocol

+ Expand

Calcium Signaling in Neuronal and Muscle Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Between day 50 and day 100, the cells were incubated at 37 °C with 4 µM of the cell-permeant calcium indicator Fluo-8 AM (Abcam). After 30 minutes of incubation, the cells were washed two times with culture medium (NB without phenol red) and left to recover for 15 min in the incubator prior to the start of any optical recording. During acquisition, the samples were kept at 37 °C with 5% CO2. Fluorescent time series images were acquired with a CSU-W1 spinning disk confocal microscope. The calcium transients were recorded for 2–3 minutes at 20 frames per second in three different conditions: without any treatment, with the addition of 10 µM Acetylcholine (Sigma Aldrich) and with addition of 10 μM Curare (Sigma Aldrich).
Analysis of the calcium activity was performed by measuring the variation of fluorescence intensity in each ROI with ImageJ (Fiji version 2.14). ROIs were detected manually, and neurons and muscle cells could be distinguished by morphology. The variation of fluorescence intensity for each individual ROI was then plotted in Prism 9 (GraphPad).

Urzi A., Lahmann I., Nguyen L.V., Rost B.R., García-Pérez A., Lelievre N., Merritt-Garza M.E., Phan H.C., Bassell G.J., Rossoll W., Diecke S., Kunz S., Schmitz D, & Gouti M. (2023). Efficient generation of a self-organizing neuromuscular junction model from human pluripotent stem cells. Nature Communications, 14, 8043.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!