Vegf c elisa kit

IHC, qRT-PCR, and Western blotting analysis were performed as previously described 25 (link). The results of IHC were scored by adding the staining intensity (0 = no, 1 = weak, 2 = moderate, 3 = strong staining) and the staining area (0 = no, 1 = less than 30%, 2 = between 30% and 60%, 3 = between 60% and 100% stained cells). The immunostaining scores (ranging from 0 to 6) were evaluated by two experienced pathologists in a blinded fashion. For statistical analysis, the immunostaining scores were evaluated, and a cut-off was determined. The samples were divided accordingly into low- and high- staining groups. For FABP5, CPT1A, and ACC1, a staining score of 4 was defined as the cut-off. For FASN, ACOX1, CA9, and HSL, a staining score of 3 was defined as the cut-off. Nuclear/cytoplasmic fractionation of MS751 cells was performed by using the PARIS Kit (Invitrogen, USA) following the manufacturer's protocols. Antibodies and working concentrations are presented in Table S5. The primer sequences used in this study are provided in Table S4. ELISA was performed by using a vascular endothelial growth factor C (VEGF-C) ELISA kit (Raybio, Norcross, GA, USA) following the manufacturer's instructions.