Left and right femoral arteries per mouse were pooled and lysed in Laemmli sample buffer (Bio-Rad) containing 5% β-mercaptoethanol. Samples were heat-denatured for 5 min and loaded on Bolt 4–12% or 12% Bis-Tris Gels (Life Technologies). After gel electrophoresis, proteins were transferred to Immobilon-FL membranes (Merck Millipore) according to standard procedures and incubated for 1 h in Odyssey Blocking Buffer (LI-COR Biosciences). Next, membranes were incubated at 4°C overnight with the following primary antibodies: rabbit anti-SQSTM1/p62 (Sigma-Aldrich, P0067) or mouse anti-β-actin (Sigma-Aldrich, A5441). Finally, membranes were incubated with fluorescently labeled secondary antibodies (LI-COR Biosciences, anti-rabbit: IgG926-3221 and anti-mouse: IgG926-68070) to allow IR-detection on an Odyssey SA instrument (LI-COR Biosciences).
Rabbit anti sqstm1 p62
Manufactured by Merck Group
Rabbit anti-SQSTM1/p62 is a primary antibody that recognizes the SQSTM1/p62 protein. SQSTM1/p62 is a multifunctional protein involved in various cellular processes. This antibody can be used in techniques such as Western blotting and immunohistochemistry to detect and study the SQSTM1/p62 protein.
Automatically generated - may contain errors
Lab products found in correlation
Odyssey blocking buffer, by LI COR (2 mentions)
Immobilon fl membrane, by Merck Group (2 mentions)
Laemmli sample buffer, by Bio-Rad (2 mentions)
Mouse anti β actin, by Merck Group (2 mentions)
Bis tris gel, by Thermo Fisher Scientific (2 mentions)
Odyssey sa instrument, by LI COR (2 mentions)
Mouse anti lc3b, by Nanotools (1 mentions)
2 protocols using rabbit anti sqstm1 p62
1
Western Blot Analysis of p62 and β-Actin
2
Western Blot Analysis of Autophagy Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Vessel segments were lysed in Laemmli sample buffer (Bio-Rad) containing 5% β-mercaptoethanol.
Samples were heat-denatured for 5 minutes and loaded on Bolt 4-12% or 12% Bis-Tris Gels (Life Technologies). After gel electrophoresis, proteins were transferred to Immobilon-FL membranes (Merck Millipore) according to standard procedures and incubated for 1 hour in Odyssey Blocking Buffer (LI-COR Biosciences). Next, membranes were incubated at 4°C overnight with the following primary antibodies: mouse anti-LC3B (Nanotools, clone 5F10, 0231-100), rabbit anti-SQSTM1/p62 (Sigma-Aldrich, P0067) or mouse anti-β-actin (Sigma-Aldrich, A5441). Finally, membranes were incubated with fluorescently-labeled secondary antibodies to allow IR-detection on an Odyssey SA instrument (LI-COR Biosciences).
Samples were heat-denatured for 5 minutes and loaded on Bolt 4-12% or 12% Bis-Tris Gels (Life Technologies). After gel electrophoresis, proteins were transferred to Immobilon-FL membranes (Merck Millipore) according to standard procedures and incubated for 1 hour in Odyssey Blocking Buffer (LI-COR Biosciences). Next, membranes were incubated at 4°C overnight with the following primary antibodies: mouse anti-LC3B (Nanotools, clone 5F10, 0231-100), rabbit anti-SQSTM1/p62 (Sigma-Aldrich, P0067) or mouse anti-β-actin (Sigma-Aldrich, A5441). Finally, membranes were incubated with fluorescently-labeled secondary antibodies to allow IR-detection on an Odyssey SA instrument (LI-COR Biosciences).
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