Three samples of primary tissue per patient were randomly selected. Paraffin-embedded slides were deparaffinized, rehydrated, microwaved in the EDTA pH 9.1 to retrieve antigens, and were then stained for CD4+FOXP3+ T cells by using the fluorescently labeled rabbit anti-human CD4 (Abcam, USA) and the mouse anti-human FOXP3 (Abcam, USA). Slides were imaged under a fluorescence microscope. Images obtained at different wavelengths were superimposed in Image Processing and Analysis in Image J (National Institute of Health, USA) to identify cells with fluorescently stained cell membranes, nuclei, or cytoplasm. CD4+FOXP3+ T cells were quantified in each sample as (cumulative optical density after correction of CD4 fluorescence index + cumulative optical density after correction of FOXP3 fluorescence index)/2.[38 (link)]
Mouse anti human foxp3
Manufactured by Abcam
Sourced in United States
Mouse anti-human FOXP3 is a primary antibody that specifically binds to the human FOXP3 protein. FOXP3 is a transcription factor that plays a crucial role in the development and function of regulatory T cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mouse anti human foxp3
1
Quantification of CD4+FOXP3+ T cells
2
Multiplex IHC Analysis of Colorectal Cancer
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Formalin fixed paraffin-embedded specimens from 70 patients diagnosed with colorectal cancer who underwent surgical resection of tumor tissue were included in this study and analyzed by multiplex fluorescent immunohistochemistry (IHC) under an approved Institutional Review Board protocol. These patients did not receive chemoradiotherapy or immunotherapy before surgery. The primary antibodies and IHC metrics were: rabbit anti-human CCL28 (Invitrogen, 1:1500), rabbit anti-human CCR10 (Proteintech, 1:800), mouse anti-human FoxP3 (Abcam, 1:500). Multiplex fluorescent staining was obtained using Opal 4-Color Manual IHC Kit (NEL810001KT, PerkinElmer). Slides were scanned and visualized using the TissueFAXSi-plus imaging system (TissueGnostics, Vienna, Austria; acquisition software: TissueFAXS v7.0.6245) equipped with a digital Pixelink color camera (PCO AG). Multispectral images were analyzed with StrataQuest software v7.0.1.165 (TissueGnostics). The correlations of different biomarkers were evaluated by Spearman's correlation analysis (P < 0.05). Kaplan–Meier Log Rank test was used to perform univariate survival analysis. SPSS (version 25.0, IBM) and GraphPad Prism (version 8.0.1, US) were used for statistical analysis.
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