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4 6 diamidino 2 phenylindole dapi

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4',6-diamidino-2-phenylindole (DAPI) is a fluorescent stain that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used in fluorescence microscopy and flow cytometry applications to visualize and quantify DNA.

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Lab products found in correlation

Dmire2, by Leica (1 mentions) Hybrite, by Abbott (1 mentions) Bx50 microscope, by Olympus (1 mentions)

2 protocols using 4 6 diamidino 2 phenylindole dapi

1

DNA FISH Analysis of Stem Cells

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For DNA FISH analysis, the hBES-1, hPES-1 and hPES-2 cells were dropped onto wet slides and dried at room temperature overnight. They were then fixed with 0.1% Tween-20 and 0.01 N HCl and dehydrated with an ethanol series at concentrations of 70, 85 and 100%. CEPX/CEPY (Cytocell, Banbury, Oxfordshire, UK) was used for hybridization for 2 h. Finally, the cells were stained with 4′,6-diamidino-2-phenylindole (DAPI; Cytocell) for 5 min. The cells were examined under a fluorescence microscope (Leica DMIRE 2; Leica Microsystems GmbH, Wetzlar, Germany). At least 20 cells were examined for each experiment.
QI Q., DING C., HONG P., YANG G., XIE Y., WANG J., HUANG S., HE K, & ZHOU C. (2014). X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging. International Journal of Molecular Medicine, 35(3), 569-578.
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2

Fluorescent In Situ Hybridization of Splenic Cells

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Several splenic regions were touched onto slides coated with poly-L-lysine, fixed and air-dried. The following FISH probes were applied: CEP10, CEP12 (Cytocell, Cambridge, Cambridgeshire, UK) and 11q (Abbott Molecular, Maidenhead, UK). Hybridisations were performed with 2 min denaturation at 73°C and 16 h incubation at 37°C on a HyBrite (Abbott Molecular) and subsequent application of 4′6-diamidino-2-phenylindole (DAPI) (Cytocell, Cambridge, Cambridgeshire, UK). Cells (>200/sample) were visualised on an Olympus BX50 microscope (Southend-on-Sea, Essex, UK).
Oldreive C.E., Skowronska A., Davies N.J., Parry H., Agathanggelou A., Krysov S., Packham G., Rudzki Z., Cronin L., Vrzalikova K., Murray P., Odintsova E., Pratt G., Taylor A.M., Moss P, & Stankovic T. (2015). T-cell number and subtype influence the disease course of primary chronic lymphocytic leukaemia xenografts in alymphoid mice. Disease Models & Mechanisms, 8(11), 1401-1412.
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