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2 protocols using hexadecanediol

1

Synthesis of Functional Nanomaterials

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Poly(maleic
anhydride-alt-1-octadecene), Mn 30 000–50 000
g/mol (Aldrich),
Milli-Q water (18.2 MΩ, filtered with filter pore size 0.22
μM) from Millipore, acetonitrile (HPLC grade, J. T. Baker) and
tetrahydrofuran anhydride (Carlo Erba, p.a.), iron oxide hydroxide
(Sigma-Aldrich, #371254), iron acetylacetonate (Sigma-Aldrich, 99%),
manganese acetylacetonate (Sigma-Aldrich, #245763), hexadecanediol
(Sigma-Aldrich, 90%), dodecylamine (Sigma-Aldrich, 98%), lauric acid
(Sigma-Aldrich, 99%), benzyl ether (Sigma-Aldrich, 98%), and octadecene
(Sigma-Aldrich, 90%), YCl3, YbCl3, ErCl3, ammonium fluoride, and sodium oleate, tri-n-octylphosphine oxide (Strem, 99%), tri-n-octylphosphine
(Strem, 97%), n-octadecylphosphonic acid (PolyCarbon
Industries, 99%), cadmium oxide (Sigma-Aldrich, 99.99%), selenium
(Sigma-Aldrich, 99.99%), diethylzinc (Sigma-Aldrich, 1 M in hexane)
and hexamethyldisilathiane (Sigma-Aldrich, synthesis grade), ethanol
(Sigma-Aldrich, 99.8%), methanol (Sigma-Aldrich, 99.9%), toluene (Sigma-Aldrich,
99.5%), and hexane (Sigma-Aldrich, 99%) were used without any further
purification. All reagents for cell cytotoxicity assay were purchased
from Gibco (ThermoFisher Scientific).
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2

Synthesis of Functionalized Magnetic Nanoparticles

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Poly(maleic anhydride-alt-1-octadecene), Mn 30,000–50,000 (Aldrich), Milli-Q
water
(18.2 MΩ, filtered with filter pore size 0.22 μM) from
Millipore, acetonitrile (HPLC grade, J. T. Baker) and tetrahydrofuran
anhydride (Carlo Erba, p.a.), iron oxide hydroxide (Sigma-Aldrich,
#371254), iron acetylacetonate (Sigma-Aldrich, 99%), manganese acetylacetonate
(Sigma-Aldrich, #245763), hexadecanediol (Sigma-Aldrich, 90%), dodecylamine
(Sigma-Aldrich, 98%), lauric acid (Sigma-Aldrich, 99%), benzyl ether
(Sigma-Aldrich, 98%), and octadecene (Sigma-Aldrich, 90%), as well
as 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide hydrochloride (EDC)
(Aldrich, commercial grade), O-(2-aminoethyl)-O′-methyl polyethylene glycol (“aminoPEG750”,
Aldrich), N,N-dimethylethylenediamine
(for “tertiary amine functionalization”, Aldrich), 2-(2-pyridyl)ethylamine
(for “pyridine functionalization”, Aldrich), and 2,2′-(ethylendedioxy)bis(ethylamine)
(referred to in this article as “diamine” Aldrich),
tetramethylammonium hydroxide (TMAOH, Sigma-Aldrich) were used without
further purification. Acetonitrile (ACN, not anhydrous) was purchased
from J. T. Baker and stored in the glovebox.
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