resuspended in media. Fibroblasts were cultured in Fibroblast Medium (ScienCell,
Carlsbad, CA USA, Cat. 2301) and epithelial cells were grown in Prostate
Epithelial Cell Medium (ScienCell, Cat. 4411). Cells were plated at 25,000 cells
per well in a standard 96 well TC treated plate (Corning) and cultured at 37C
with 5% CO2. After 24 hours, media was removed and replaced. Media
changes were then performed every 3 days.