Apoptosis was examined using an Annexin V-FITC-PI Dual Staining Kit (Biolegend, San Diego, CA, USA) followed by flow cytometry analysis as per the manufacturer’s instructions. Briefly, SKMEL-28 and WM1552C cells were transfected with miR-26a mimics (50 or 100 μM) or negative control microRNA mimics for 48 h and harvested by trypsinization, washed with ice-cold PBS, and resuspended in binding buffer at a density of 1×106 cells per ml. Cell suspension was stained with Annexin V and PI and analyzed by the Accuri C6 Flow Cytometer System.
Annexin 5 fitc pi dual staining kit
Manufactured by BioLegend
Sourced in United States
The Annexin V-FITC-PI Dual Staining Kit is a laboratory reagent used to assess apoptosis in cells. It contains Annexin V conjugated to the fluorescent dye FITC and the DNA stain propidium iodide (PI). The kit allows for the simultaneous detection of apoptotic and necrotic cells through flow cytometric analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using annexin 5 fitc pi dual staining kit
1
Apoptosis Analysis by Flow Cytometry
2
Apoptosis Evaluation by Annexin V-FITC-PI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis was assessed using an annexin V-FITC-PI dual staining kit (Biolegend, USA) followed by flow cytometry analysis per manufacturer's instructions. Briefly, the cell pellet (5×103 cells) was resuspended in 500 μL binding buffer. Next, 5 μL each of annexin V-FITC and PI were added to the cell suspension, and the cells were cultured in the dark for 15 min at room temperature, followed by flow cytometry analysis (BD Biosciences). Each experiment was repeated thrice.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!