Cells grown on coverslips were fixed in 4% paraformaldehyde and permeabilized in PBS with 0.2% Triton X-100. After washing, cells were blocked in 3% BSA. Primary Mcl-1 (sc819, Santa Cruz), WT1 (NBP2–47858PE, NOVUS) conjugated with PE, pan-Cytokeratin (sc-8018, Santa Cruz) conjugated with PE, Vimentin (sc-66002, Santa Cruz) conjugated with FITC antibodies and TOMM20 (ab209606, Abcam) antibodies conjugated with Alexa Fluor 647 were diluted in blocking solution and incubated overnight at 4°C. DAPI was used as internal control for immunofluorescence. Images were collected with a Zeiss 780 confocal microscope.
Pan cytokeratin sc 8018
Manufactured by Santa Cruz Biotechnology
Pan-Cytokeratin (sc-8018) is a primary antibody that recognizes a broad range of cytokeratin proteins. Cytokeratins are intermediate filament proteins found in the intracytoplasmic cytoskeleton of epithelial tissue.
Automatically generated - may contain errors
2 protocols using pan cytokeratin sc 8018
1
Multi-Marker Immunofluorescence Imaging
2
Multiparametric Immunofluorescence Analysis
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Cells grown on coverslips were fixed in 4% PFA and permeabilized in PBS with 0.2% Triton X-100. After washing, cells were blocked in 3% BSA. Primary Mcl-1 (sc819, Santa Cruz Biotechnology), WT1 (NBP2-47858PE, NOVUS) conjugated with PE, pan-cytokeratin (sc-8018, Santa Cruz Biotechnology) conjugated with PE, Vimentin (sc-66002, Santa Cruz Biotechnology) conjugated with FITC antibodies and TOMM20 (ab209606, Abcam) antibodies conjugated with Alexa Fluor 647 were diluted in blocking solution and incubated overnight at 4°C. DAPI was used as internal control for immunofluorescence. Images were collected with a Zeiss 780 confocal microscope.
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