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The BMS228 is a laboratory instrument designed for biomolecular analysis. It provides core functionality for researchers working in the field of molecular biology and biochemistry.

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Lab products found in correlation

Bms223 4, by Thermo Fisher Scientific (2 mentions) Microplate reader, by Bio-Rad (2 mentions) Eh2il2, by Thermo Fisher Scientific (1 mentions) Corresponding kits, by Thermo Fisher Scientific (1 mentions) Khc3011, by Thermo Fisher Scientific (1 mentions) Bms215 2, by Thermo Fisher Scientific (1 mentions) Bms221 2, by Thermo Fisher Scientific (1 mentions)

4 protocols using bms228

1

Exosomal PD-L1 and T-cell Cytokines

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PD‐L1 concentration on exosomes was analyzed by serial dilution, and the concentration of PD‐L1 in exosomes derived from cells or plasma was calculated based on ELISA analysis. The CD8+ T‐cell supernatant was collected to detect the contents of IFN‐γ (#BMS228), TNF‐α (#KHC3011), and interleukin (IL)‐2 (#EH2IL2) following the protocols of corresponding kits (Thermo Fisher Scientific).
Yang J., Chen J., Liang H, & Yu Y. (2022). Nasopharyngeal cancer cell‐derived exosomal PD‐L1 inhibits CD8+ T‐cell activity and promotes immune escape. Cancer Science, 113(9), 3044-3054.
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2

Cytokine Profiling of CAR T Cells

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CAR T cells or control T cells were cocultured with target cells (K562-PDL1-GL, HeLa-GL or H460-MSLN-GL cells) at a 1:1 E:T ratio for 24 h, and then the culture supernatants were collected. The production of IL-2 (ThermoFisher, BMS221-2), IFN-γ (ThermoFisher, BMS228), IL5 (ThermoFisher, BMS278), IL10 (ThermoFisher, BMS215-2) and IL13 (ThermoFisher, BMS231-3) was measured with enzyme-linked immunosorbent assay (ELISA) kits. All ELISAs were performed according to the manufacturer’s protocols.
Qin L., Cui Y., Yuan T., Chen D., Zhao R., Li S., Jiang Z., Wu Q., Long Y., Wang S., Tang Z., Pan H., Li X., Wei W., Yang J., Luo X., Zhang Z., Tang Q., Liu P., Weinkove R., Yao Y., Qin D., Thiery J.P, & Li P. (2022). Co-expression of a PD-L1-specific chimeric switch receptor augments the efficacy and persistence of CAR T cells via the CD70-CD27 axis. Nature Communications, 13, 6051.
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3

Quantification of Cytokine Levels

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The levels of IFN‐γ and TNF‐α in the cell culture medium were quantified utilizing ELISA kits (BMS228 and BMS223‐4, Invitrogen). In summary, the culture medium was collected and centrifuged at 1400 rpm for 1 min. The ELISA assay was conducted according to the provided instructions, and the absorbance at 450 nm (A450) was measured using a microplate reader (Bio‐Rad).
Wei L., He P., Tan Z., Zhao L., Lin C, & Wei Z. (2024). Unveiling the role of the KLF4/Lnc18q22.2/ULBP3 axis in the tumorigenesis and immune escape of hepatocellular carcinoma under hypoxic condition. Journal of Cellular and Molecular Medicine, 28(10), e18411.
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4

Quantifying IFN-γ and TNF-α Levels

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The levels of IFN‐γ and TNF‐α in the cell culture medium were quantified using ELISA kits (BMS228 and BMS223‐4, Invitrogen). Briefly, the culture medium was collected and centrifuged at 1400 rpm for 1 min. The ELISA assay was performed according to the kit instructions, and A450 was determined using a microplate reader (Bio‐Rad).
Xiang T., Li Y., Liu G, & Li X. (2024). NR1D1‐transactivated lncRNA NUTM2A‐AS1 promotes chemoresistance and immune evasion in neuroblastoma via inhibiting B7‐H3 degradation. Journal of Cellular and Molecular Medicine, 28(10), e18360.
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