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Anti human cd29

Manufactured by BD
Sourced in United States

Anti-human CD29 is a laboratory reagent used for the identification and characterization of cells expressing the CD29 (Integrin beta-1) antigen. CD29 is a cell surface glycoprotein involved in cell-cell and cell-matrix interactions. This product can be used in flow cytometry, immunohistochemistry, and other applications to detect the presence of CD29-positive cells.

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2 protocols using anti human cd29

1

Characterization of LPC Cell Antigens

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The specific cell surface antigens of LPCs were characterized by flow cytometry. The cells were incubated with fluorescence-conjugated antibodies at the recommended concentration for 30 min in ice. All the antibodies were obtained from BD biosciences, including anti-human CD29, CD44, CD73, CD90, CD34, CD31, CD45, anti-mouse CD44, CD31, CD34. Data analysis was performed with FlowJo software (FlowJo, Ashland, OR).
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2

Characterization of hAMSC and hAEC Surface Antigens

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hAMSC and hAEC specific surface antigens were stained with PE-conjugated antibodies of anti-human-CD-105, anti-human-CD-29, anti-human-CD-44, anti-human-CD-73, anti-human-CD-90, anti-human-CD-34, anti-human-CD-45, anti-human-EpCam, or anti-human-CD-166 (BD, USA) or their corresponding isotype control. The stained cells were then analyzed respectively by fluorescence-activated cell sorting (FACS). The detailed instruction is the same as the FACS analysis already described. Differentiation ability of hAMSCs was tested using hAMSC differentiation kits for culture (Thermo, USA).
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