Lipofectamine 3000 reagent

hiPSC-NSCs and hiPSC neurons, which had been differentiated for 2 to 3 weeks in well plates (CELLSTAR^®, Greiner), were: (i) transfected with 2 µg/mL poly(dA:dT) (InvivoGen, cat n° tlrl-patn) using Lipofectamine^TM 3000 Reagent (Invitrogen, cat n° L3000001) for 24 h or treated with Lipofectamine^TM 3000 Reagent alone for 24 h as a control, (ii) stimulated with 10 µg/mL poly(I:C) (InvivoGen, cat n° tlrl-pic) for 24 h without transfection, (iii) stimulated with 10 µg/mL poly(I:C) (InvivoGen, cat n° tlrl-pic) using Lipofectamine^TM 3000 Reagent for 24 h or treated with Lipofectamine^TM 3000 Reagent alone for 24 h as a control, (iv) inoculated with 1.5 × 10² PFU eGFP-ORF23 VZV lysate or a non-infected cell debris control for either 24 h or 72 h, or (v) inoculated with ARPE-19-associated eGFP-ORF23 VZV at 1:5 ratio or a non-infected ARPE-19 control for 72 h, or (vi) inoculated with Sendai virus (SeV) at MOI 0.1, 1, or 10 for 24 h. For the inoculation of hiPSC-NSCs and hiPSC neurons with SeV, the SeV-GFP4 strain (SKU: S124, ViraTree) was used. In this strain, GFP is expressed from transcription of the GFP gene which is located between the M and F genes of SeV. Cells were harvested using DNA/RNA shield (Zymo Research) and stored at −20 °C for downstream RTqPCR analysis.