Apy 29

Manufactured by Bio-Techne

The APY-29 is a laboratory device designed for the analysis and quantification of biological samples. It utilizes advanced technology to perform accurate and reliable measurements, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation on the intended use. Further information from the manufacturer would be required to offer a more comprehensive overview of the product's core function.

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Lab products found in correlation

2 protocols using apy 29

Modulation of IRE1α and PERK Signaling

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STF-083010 (IRE1α antagonist, 4509, Tocris), CCT020312 (PERK agonist, 324879, Sigma-Aldrich) and APY-29 (IRE1α agonist, 4865, Tocris) were dissolved and prepared according to manufacturer's recommendations. CCT020312 was used at 10 μM. STF-083010 and APY-29 were tested at final concentrations of 20 μM or 60 μM. The best dose was determined to be 20 μM for both drugs (cell viability quantification data not shown).

Doycheva D., Xu N., Kaur H., Malaguit J., McBride D.W., Tang J, & Zhang J.H. (2019). Adenoviral TMBIM6 vector attenuates ER-stress-induced apoptosis in a neonatal hypoxic-ischemic rat model. Disease Models & Mechanisms, 12(11), dmm040352.

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Characterization of CFTR Regulatory Pathways

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Thapsigargin (Sigma-Aldrich, T9033), STF-083010 (TOCRIS, 4509), APY29 (TOCRIS, 4865), MSC 2032964A (TOCRIS, 5641), VX-809 (Selleckchem, Chemical Abstracts Service registry no. 936727-05-8), VX-770 (Selleckchem, CAS registry no. 873054-44-5), and cycloheximide (Sigma-Aldrich, C4859) were purchased commercially. CSTMP was custom-synthesized by Cayman Chemical (Ann Arbor, MI, USA) (CAS registry no. 1000672-89-8).
The following antibodies were acquired commercially: anti-CFTR M3A7 (Millipore, Billerica, MA, USA), anti-IRE1α (Cell Signaling Technology, 3294), anti–phospho-S724 IRE1α (Abcam, ab48187), anti-XBP1 (Abcam, ab198999), anti-ASK1 (Cell Signaling Technology, 3762), anti–phospho-Thr⁸⁴⁵ ASK1 (Cell Signaling Technology, 3765), anti-BiP (Cell Signaling Technology, 3177), anti-CHOP (Cell Signaling Technology, 2895), anti-pro/p17–caspase 3, anti–cleaved PARP1 (Abcam, ab136812), anti–Na- and K-dependent ATPase (Na,K-ATPase) α1 (Cell Signaling Technology, 23565), anti-HA (Cell Signaling Technology, 2367), anti-Myc (Cell Signaling Technology, 2276), anti–aldolase A (Abcam, ab78339) anti–β-actin (Santa Cruz Biotechnology, sc47778), and anti-pendrin (Santa Cruz Biotechnology, sc23779). The anti-R4 polyclonal antibody was raised against peptides corresponding to amino acids 1458 to 1471 of human CFTR, as described previously (12 (link)).

Park H., Shin D.H., Sim J.R., Aum S, & Lee M.G. (2020). IRE1α kinase–mediated unconventional protein secretion rescues misfolded CFTR and pendrin. Science Advances, 6(8), eaax9914.

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