Goat anti guinea pig igg hrp

Manufactured by Thermo Fisher Scientific

Sourced in United States

Goat anti-Guinea Pig IgG-HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed for the detection of guinea pig immunoglobulin G (IgG) in various immunoassay applications.

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Lab products found in correlation

Goat anti mouse igg hrp, by Thermo Fisher Scientific (2 mentions) Rabbit da1e mab igg xp isotype control, by Cell Signaling Technology (1 mentions) Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 594 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Anti gfp, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Abcam (1 mentions) Tmb substrate, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Goat anti-guinea pig IgG (4 citations)

3 protocols using goat anti guinea pig igg hrp

Autophagy and Nutrient Sensing Signaling Pathway Analysis

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AMPKα (D5A2) Rabbit mAb (#5831, 1:1000), phospho- AMPKα (Thr172) (40H9) Rabbit mAb (#2535, 1:500), Raptor (24C12) Rabbit mAb (#2280, 1:500), phospho-Raptor (Ser792) Rabbit polyclonal antibody (pAb) (#2083, 1:500), mTOR (7C10) Rabbit mAb (#2983, 1:500), phospho-mTOR (Ser2481) Rabbit pAb (#2974, 1:500), 4E-BP1 (#9452, 1:500), phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (#2855, 1:1000), ULK1 (D8H5) Rabbit mAb (#8054, 1:500), FIP200 (D10D11) Rabbit mAb (#12436, 1:250), LC3B Rabbit pAb (#2775, 1:1000) and Rabbit (DA1E) mAb IgG XP® Isotype Control (#3900) were from Cell Signaling Technology. Goat anti-Rabbit IgG-HRP pAb (sc-2004, 1:1000) were from Santa Cruz Biotechnology, Inc. NRBF2 Rabbit pAb (A301-851A) was from Bethyl Laboratories, Inc. P62 Guinea pig pAb (#GP62-C, 1:4000) was from PROGEN. P62 Guinea pig pAb (PM066, 1:4000) was from Medical and Biological Laboratories Co., LTD. (MBL). Goat anti-Mouse IgG-HRP (#A28177, 1:1000), goat anti-Guinea Pig IgG-HRP (#A18775, 1:1000) antibodies were from Thermo Scientific. PSD95 (6G6-1C9) mAb (#MA1–045, 1:1000) was from Thermo Scientific. VAMP2 Rabbit pAb (#104202, 1:5000) was from Synaptic Systems.

Lachance V., Wang Q., Sweet E., Choi I., Cai C.Z., Zhuang X.X., Zhang Y., Jiang J.L., Blitzer R.D., Bozdagi-Gunal O., Zhang B., Lu J.H, & Yue Z. (2019). Autophagy protein NRBF2 has reduced expression in Alzheimer’s brains and modulates memory and amyloid-beta homeostasis in mice. Molecular Neurodegeneration, 14, 43.

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Antibody Panel for Viral Protein Detection

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Anti-β-actin (Abcam, # ab8227, WB (1:1,000)), anti-FLAG (Sigma, # F3165, WB (1:1,000), IFA (1:100)), anti-GFP (Santa Cruz Biotechnology, # sc-9996, WB (1:2,000)), anti-HA epitope (Sigma, # H3663, WB (1:1,000)), anti-NP (BEI resources, # NR-4282, WB (1:1,000), IFA (1:100)), anti-NP (EMD Millipore, # MAB8800, IFA (1:1,000)), anti-HA from A/California/04/2009 (H1N1) (BEI resources, # NR-42021, IFA (1:100)), anti-influenza A/Aichi/2/68 (BEI resources, # NR-3125, IFA (1:100)), anti-PB1 (BEI resources, # NR-31690, WB (1:1,000)), anti-PB2 (Genetex, # GTX125926, WB (1:1,000)), anti-PA (Genetex, # GTX125932, WB (1:1,000)), anti-PKP2 (Fitzgerald Industries International, # 20R-2656, WB (1:1,000), IFA (1:100)), anti-CPSF4 (Millipore, # MABE620, WB (1:1,000)).
Goat anti-Mouse IgG-HRP (Santa Cruz Biotechnology, # sc-2055, WB (1:10,000)), Goat anti-Rabbit IgG-HRP (Santa Cruz Biotechnology, # sc-2030, WB (1:10,000)), Goat anti-Guinea Pig IgG-HRP (Thermo Fisher Scientific, # A18775, WB (1:10,000)), Alexa Fluor 594 Goat Anti-Mouse IgG (H+L) (Life Technologies, # A11005, IFA (1:200)), Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) (Life Technologies, # A11034, IFA (1:200)), Alexa Fluor 488 Goat Anti-Guinea Pig IgG (H+L) (Life Technologies, # A11073, IFA (1:200)).

Wang L., Fu B., Li W., Patil G., Liu L., Dorf M.E, & Li S. (2017). Comparative influenza protein interactomes identify the role of plakophilin 2 in virus restriction. Nature Communications, 8, 13876.

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Quantifying Ag-specific Antibodies in Immunized Animals

Cited 1 time

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To determine Ag-specific antibodies in the immunized and tick immune animals, ELISA was performed as described previously (Narasimhan et al., 2020 (link)). Briefly, 96-well plates were coated overnight at 4 °C with 200 ng of saliva or recombinant tick salivary lectin pathway inhibitor (TSLPI) diluted in carbonate-bicarbonate buffer, pH 9.6 (Narasimhan et al., 2020 (link)). Plates were washed and blocked in PBS with 3% BSA and 0.02% Tween 20. Guinea pig and mouse sera were serially diluted and incubated in the wells for 2 hours and 37 °C. The sera dilutions utilized for ELISA are indicated on the figures. Wells were washed, and reactivity was detected using goat anti-guinea pig IgG-HRP (ThermoFisher, Waltham, MA, USA catalog # A18769; 1:10,000 dilution) or goat anti-mouse IgG-HRP (ThermoFisher, Waltham, MA, USA; catalog # 31430; 1:2000 dilution) and TMB substrate (ThermoFisher, Waltham, MA, USA).

Kurokawa C., Narasimhan S., Vidyarthi A., Booth C.J., Mehta S., Meister L., Diktas H., Strank N., Lynn G.E., DePonte K., Craft J, & Fikrig E. (2020). Repeat tick exposure elicits distinct immune responses in guinea pigs and mice. Ticks and tick-borne diseases, 11(6), 101529.

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