Cells were grown to confluence in 24-well plates. After the indicated treatments, the cells were washed with PBS and fixed with 4% paraformaldehyde (PFA). Then the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated VCAM-1 antibody, a PE-conjugated ICAM-1 antibody, or a PE-conjugated E-selectin antibody (BD Biosciences, Franklin Lakes, NJ, USA) for 48 h. After that, the cell nuclei were counterstained with DAPI (Beyotime Biotechnology, China) for 15 min. The fluorescent signal was detected with a confocal microscope (Nikon, Japan). Image-Pro Plus 6.0 software (Media Cybernetics, Bethesda, MD, USA) was used to calculate the immunofluorescence light intensity. The average light intensity was obtained by summation of light intensity divided by the area of interest.
Fitc conjugated vcam 1 antibody
Manufactured by BD
Sourced in United States
The (FITC)-conjugated VCAM-1 antibody is a laboratory reagent used for the detection and study of the Vascular Cell Adhesion Molecule-1 (VCAM-1) protein. The antibody is labeled with the fluorescent dye FITC (Fluorescein Isothiocyanate), which allows for the visualization and quantification of VCAM-1 expression in various experimental applications.
Automatically generated - may contain errors
2 protocols using fitc conjugated vcam 1 antibody
1
Endothelial Cell Adhesion Molecule Imaging
2
VCAM-1 Expression in pHUVECs and HUVSMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells (pHUVECs and HUVSMCs) were washed with PBS and fixed with 4% paraformaldehyde. Cells (pHUVECs and HUVSMCs) were incubated with a fluorescein isothiocyanate (FITC)-conjugated VCAM-1 antibody (BD, Franklin Lakes, NJ, USA) for 30 min, and then the cell nuclei were counterstained with DAPI (Beyotime Biotechnology, Jiangsu, China) for 15 min. The fluorescent signal was detected with a confocal microscope (Nikon, Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!