The apoptosis of HepG2 cells treated with extracted scolopentide was determined by Annexin V-Alexa Fluor660/7-AAD (BB20121, BestBio, China). The cells were digested and collected by trypsin. Approximately 5 × 105 cells were collected after washing with PBS twice and centrifuged at 2000 rpm for 5 min. Then, 100 μL of Annexin V binding buffer was added to resuspend the cells, and the concentration was adjusted to 3 × 106 cells/mL. Five microliters of annexin V-FITC and 5 μL of propidium iodide were added to the cell suspension, mixed and incubated for 10 min at 4 °C in the dark. Then, 400 μL of PBS was added to the cell suspension and mixed. Within 1 h, flow CytoFlex (A00-1-1102, Beckman, United States) was utilized to detect cell apoptosis.
Flow cytoflex
Manufactured by Beckman Coulter
Sourced in United States
The Beckman Coulter Flow CytoFlex is a flow cytometry instrument designed for the analysis of cells and particles. It utilizes laser-based technology to detect and measure the physical and fluorescent characteristics of individual cells or particles within a sample.
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Lab products found in correlation
2 protocols using flow cytoflex
1
Annexin V Apoptosis Assay for HepG2 Cells
2
Pax7 Expression in Stem Cells
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To assess stem phenotype conversion, cells were resuspended in 200 μL PBS and incubated with anti-Pax7-BV510 (4 μl/ ml) at 4° C for 1 hour. After the cells were washed three times with PBS and suspended in 100 μL PBS, the analysis was performed on a Flow CytoFLEX (Beckman Coulter, USA).
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