Egg phosphatidylcholine pc

Egg phosphatidylcholine (PC) and Na-salt N-(carbonyl-methoxypolyethylen glycol-2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (PEG₂₀₀₀-DSPE) were purchased from Lipoid GmbH, Ludwigshafen, Germany. Cholesterol (Chol) was purchased from Carlo Erba Reagent, Ronado, Italy. Tween 20 was purchased from Ajax Finechem, Auckland, New Zealand. NaFI and d-limonene were purchased from Sigma-Aldrich, St Louis, MO, USA. Lissamine™ rhodamine B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (Rh-PE) was purchased from Invitrogen, Carlsbad, CA, USA.

Phospholipid (phosphatidylcholine) was extracted from chicken egg yolk in the laboratory. Egg phosphatidylcholine (PC, purity > 96.5%) was obtained from Lipoid (Germany) to be used as a reference standard in the UHPLC analysis of extracted egg phospholipid (EP). Acetonitrile and HPLC grade Methanol were purchased from BDH Chemicals Ltd. (U.K.).
Black seed oil was bought from local market (Bangladesh). For quantification of amount of thymoquinone present in black seed oil, thymoquinone and Potassium dihydrogen phosphate were procured from Sigma-Aldrich (USA) and Winlab Ltd (UK) respectively. Cholesterol (Ch) was purchased from LobaChemie (India). Methanol, ethanol, dicholoromethane, acetone, BHT (Butylatedhydroxytoluene), sodium dihydrogen phosphate, and poly-ethylene glycol 6000 (PEG600) were obtained from Merck (Germany). Sodium chloride (NaCl) was obtained from Sigma-Aldrich (USA). All other reagents were of analytical grade and used without further purification.