Cells were lysated in radioimmunoprecipitation assay lysis buffer supplemented with a protease inhibitor cocktail (Sigma-Aldrich). Protein samples were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes. The membranes were blocked with 5% fat-free milk at room temperature for 2 h, followed by overnight incubation with primary antibodies at 4°C. The primary antibodies included: anti-TET1, anti-TET2, anti-TET3, and anti-GAPDH (all from Novus Biologicals, Littleton, CO, USA). The membranes were then incubated with secondary antibodies (Cell Signaling Technology, Waltham, MA, USA). Signals were visualized using the Pierce Enhanced Chemiluminescence (ECL) Western Blotting Substrate (Thermo Fisher Scientific, Rockford, IL, USA).
Anti tet3
Manufactured by Novus Biologicals
Sourced in United States, China
Anti-TET3 is a laboratory reagent used to detect and measure the expression of the TET3 protein. TET3 is an enzyme involved in DNA methylation. Anti-TET3 can be used in various research applications that require the analysis of TET3 expression levels.
Automatically generated - may contain errors
Lab products found in correlation
Anti tet1, by Novus Biologicals (2 mentions)
Anti gapdh, by Novus Biologicals (1 mentions)
Pierce enhanced chemiluminescence ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Horseradish peroxidase hrp conjugated anti rabbit secondary antibody, by Cell Signaling Technology (1 mentions)
Anti anxa1, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti eno1, by Cell Signaling Technology (1 mentions)
Anti pten, by Cell Signaling Technology (1 mentions)
Anti krt19, by Cell Signaling Technology (1 mentions)
Anti cd9, by Cell Signaling Technology (1 mentions)
Anti tet2, by Cell Signaling Technology (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti cd63, by Abcam (1 mentions)
Anti tsg101, by Abcam (1 mentions)
Anti cd81, by Abcam (1 mentions)
2 protocols using anti tet3
1
Western Blot Analysis of TET Proteins
2
Exosome Protein Profile Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cells were collected, washed twice with PBS, and lysed in RIPA buffer containing protease inhibitors. After determining the protein concentration with a BCA Protein Assay Reagent Kit (Pierce), equal amounts of protein were separated on 8% SDS-PAGE, electrically transferred to PVDF membrane, and blocked with 5% skim milk. The membranes were incubated with anti-CD63 (1:500; Abcam, China), anti-TSG101 (1:800; Abcam), anti-CD81 (1:1000; Abcam), anti-CD9 (1:1000; Cell Signaling Technology, China), anti-ENO1 (1:800; Cell Signaling Technology), anti-KRT19 (1:800; Cell Signaling Technology), anti-ANXA1 (1:800; Cell Signaling Technology), anti-PTEN (1:800; Cell Signaling Technology, China), anti-TET1 (1:500; Novus Biologicals, China), anti-TET2 (1:500; Cell Signaling Technology), anti-TET3 (1:500; Novus Biologicals), anti-Akt (1:1000; Cell Signaling Technology), anti-p-Akt (1:1000; Cell Signaling Technology) or anti-beta-actin (1:1000; Cell Signaling Technology) primary antibody overnight at 4 °C. After washing, the membranes were then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody (Cell Signaling Technology) at room temperature for 1 h. Finally, the membranes were incubated with West Femto chemiluminescence substrate (Pierce), and images were visualized and recorded.
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