Anti-mouse CD47 (clone miap301; BioLegend), anti-human CD47 (clone B6H12; BD Biosciences), anti-mouse F4/80 (clone BM8; BioLegend), anti-mouse/human CD11b (clone M1/70; BioLegend), anti-mouse MHC-II (clone M5/114.15.2; BioLegend), anti-mouse CD206 (clone C068C2; BioLegend), anti-mouse Sirpα (clone P84; BioLegend) and anti-human CD206 (clone 15-2; BioLegend) were used for FACS analyses. Antibodies were phycoerythrin (PE), PE Cy7, APC, APC Cy7, Alexa Fluor 700, BV787, or BV605 conjugated, or fluorophore-conjugated secondary antibodies were used. Sytox blue was used to exclude dead cells. Annexin V (BD Biosciences) and 7-aminoactinomycin D (7-AAD; ThermoFisher) were used to evaluate cell viability of macrophages on cabazitaxel treatment. Flow cytometry was performed using the BD LSRFortessa cell analyzers.
Anti mouse cd206 clone c068c2
Manufactured by BioLegend
Anti-mouse CD206 (clone C068C2) is a monoclonal antibody that binds to the mouse CD206 antigen. CD206, also known as the mannose receptor, is a cell surface receptor that recognizes and binds to carbohydrates found on the surface of pathogens.
Automatically generated - may contain errors
Lab products found in correlation
Lsrfortessa cell analyzer, by BD (3 mentions)
Anti mouse human cd11b clone m1 70, by BioLegend (3 mentions)
Anti mouse f4 80 clone bm8, by BioLegend (3 mentions)
Anti mouse mhcii clone m5 114, by BioLegend (3 mentions)
7 aminoactinomycin d 7 aad, by Thermo Fisher Scientific (2 mentions)
Annexin 5, by BD (2 mentions)
Anti human cd47 clone b6h12, by BD (2 mentions)
Anti mouse sirpα clone p84, by BioLegend (1 mentions)
Sytox blue, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd86 clone gl 1, by BioLegend (1 mentions)
Zombie violet fixable viability kit, by BioLegend (1 mentions)
Anti mouse cd80 clone 16 10a1, by BioLegend (1 mentions)
Anti mouse cd45 clone 30 f11, by BioLegend (1 mentions)
Clone m a712, by BD (1 mentions)
Antimouse gr1 clone rb6 8c5, by BioLegend (1 mentions)
Anti mouse pd l1 clone 10f 9g2, by BioLegend (1 mentions)
3 protocols using anti mouse cd206 clone c068c2
1
Multiparameter Flow Cytometry Analysis
2
Multiparametric Flow Cytometry Analyses
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Anti-mouse F4/80 (clone BM8, BioLegend), anti-mouse/human CD11b (clone M1/70, BioLegend), anti-mouse MHCII (clone M5/114.15.2, BioLegend), anti-mouse iNOS (clone W16030C, BioLegend), anti-mouse CD206 (clone C068C2, BioLegend), and anti-mouse CSF1R (clone AFS98, BioLegend) were used for FACS analyses. The antibodies were PE-, PE-Cy7–, allophycocyanin (APC)–, APC Cy7–, or Alexa Fluor 700–conjugated, or fluorophore-conjugated secondary antibodies were used. Sytox blue or Zombie Violet was used to exclude dead cells. Flow cytometry was performed using the BD LSRFortessa cell analyzers.
3
Comprehensive Immune Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-human CD47 (clone B6H12, BD Biosciences), anti-mouse F4/80 (clone BM8, BioLegend), anti-Sirpα (clone P84, BioLegend), anti-mouse/human CD11b (clone M1/70, BioLegend), anti-mouse CD45 (clone 30-F11, BioLegend), anti-mouse MHC II (clone M5/114.15.2, BioLegend), anti-mouse CD206 (clone C068C2, BioLegend), anti-mouse CD80 (clone 16-10A1, BioLegend), anti-mouse CD86 (clone GL-1, BioLegend), anti-mouse PD-L1 (clone 10F.9G2, BioLegend), anti-mouse Gr1(clone RB6-8C5,BioLegend), anti-human CD14 (clone HCD14, BioLegend), and anti-human CD71 (clone CY1G4, BioLegend; clone OKT9, ThermoFisher; clone L01.1 and clone M-A712, BD Biosciences) were used for FACS analyses. Antibodies were Phycoerythrin (PE)-, PE/Cyanine7, APC, APC/Cyanine7, PerCP/Cyanine5.5, PE/Dazzle™ 594, Alexa Flour® 700 or BV605 conjugated, or fluorophore-conjugated secondary antibodies were used. Annexin V (BD Biosciences), Sytox blue (ThermoFisher), 7-Aminoactinomycin D (7-AAD, ThermoFisher), or Zombie Violet™ Fixable Viability Kit (Biolegend) was used to exclude dead cells. Flow cytometry was performed using the BD LSRFortessa cell analyzers (BD).
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