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Mouse anti human ccr7

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Mouse anti-human CCR7 is a primary antibody that binds to the human CC chemokine receptor 7 (CCR7). CCR7 is a G protein-coupled receptor that plays a key role in the trafficking and homing of lymphocytes.

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Lab products found in correlation

Anti human cd4, by BD (2 mentions) Human anti human cd14 microbeads, by Miltenyi Biotec (2 mentions) Cd45ra, by BD (2 mentions) Cd45ro, by BD (2 mentions) Ficoll, by GE Healthcare (2 mentions)

2 protocols using mouse anti human ccr7

1

Immune Profiling of Axial Spondyloarthritis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Axial SpA patients -fulfilling the Assessment of SpondyloArthritis International Society classification criteria (12 (link))-, age/sex-matched healthy controls (HCs) and rheumatoid arthritis (RA) patients -fulfilling the American College of Rheumatology/European League Against Rheumatism classification criteria (13 (link))- were recruited in the department of rheumatology of Ambroise Pare hospital. Clinical data are summarized in Supplementary Table S1. Peripheral venous blood peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll (GE Healthcare, Chicago - Illinois/US) density gradient centrifugation. CD14+ cells were removed by positive magnetic selection (AutoMacs) using human anti-human CD14 microbeads (Miltenyi). Total CD14− PBMC were used for ex-vivo (p)STAT1/3 assay. CD4+CD45RAhighCD45ROlowCCR7+ Tn were sorted by flow cytometry (Supplementary Figure S1D) for quantitative real-time polymerase chain reaction (q-RT-PCR). Anti-human CD4, CD45RA, CD45RO, and mouse anti-human CCR7 were from BD. Written informed consent was obtained before study, approved by the local ethical committee (Comité de Protection des Personnes Ile-de-France XI, AOR10006-NI09031).
Cherqaoui B., Crémazy F., Lauraine M., Shammas G., Said-Nahal R., Mambu Mambueni H., Costantino F., Fourmont M., Hulot A., Garchon H.J., Glatigny S., Araujo L.M, & Breban M. (2023). STAT1 deficiency underlies a proinflammatory imprint of naive CD4+ T cells in spondyloarthritis. Frontiers in Immunology, 14, 1227281.
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2

Immune Profiling of Axial Spondyloarthritis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Axial SpA patients -fulfilling the Assessment of SpondyloArthritis International Society classification criteria (12 (link))-, age/sex-matched healthy controls (HCs) and rheumatoid arthritis (RA) patients -fulfilling the American College of Rheumatology/European League Against Rheumatism classification criteria (13 (link))- were recruited in the department of rheumatology of Ambroise Pare hospital. Clinical data are summarized in Supplementary Table S1. Peripheral venous blood peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll (GE Healthcare, Chicago - Illinois/US) density gradient centrifugation. CD14+ cells were removed by positive magnetic selection (AutoMacs) using human anti-human CD14 microbeads (Miltenyi). Total CD14− PBMC were used for ex-vivo (p)STAT1/3 assay. CD4+CD45RAhighCD45ROlowCCR7+ Tn were sorted by flow cytometry (Supplementary Figure S1D) for quantitative real-time polymerase chain reaction (q-RT-PCR). Anti-human CD4, CD45RA, CD45RO, and mouse anti-human CCR7 were from BD. Written informed consent was obtained before study, approved by the local ethical committee (Comité de Protection des Personnes Ile-de-France XI, AOR10006-NI09031).
Cherqaoui B., Crémazy F., Lauraine M., Shammas G., Said-Nahal R., Mambu Mambueni H., Costantino F., Fourmont M., Hulot A., Garchon H.J., Glatigny S., Araujo L.M, & Breban M. (2023). STAT1 deficiency underlies a proinflammatory imprint of naive CD4+ T cells in spondyloarthritis. Frontiers in Immunology, 14, 1227281.
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