As previously described [22 (link)], protein extraction was conducted using RIPA buffer (Sigma‐Aldrich, USA). Equal amounts of protein samples were separated using 12% SDS‐polyacrylamide gel electrophoresis. When the protein separation was complete, polyvinylidene difluoride membranes (Invitrogen, USA) were used for the protein transfer. Next, the samples were blocked in 5% skim milk (2 h). When the block was completed, anti-UBE2T (1;1000, Cat#: ab154022, Abcam, UK), anti-ERK, anti-p-ERK, anti-RSK2, anti-p-RSK2 (1:800; Cell Signaling Technology, Danvers, MA, USA), anti-β-actin (1:1000; Abcam, Cambridge, MA), and anti‐GAPDH (1;1000, Cat#: ab8245, Abcam, UK) were used to treat the samples for overnight at 4°C. The next morning, the samples were treated with a secondary antibody (1 h). At the end, protein bands were observed with an Odyssey instrument (Li-cor, USA).
Anti p rsk2
Manufactured by Cell Signaling Technology
Sourced in United Kingdom, United States
Anti-p-RSK2 is a primary antibody that specifically recognizes the phosphorylated form of ribosomal S6 kinase 2 (RSK2). RSK2 is a serine/threonine kinase that plays a key role in the regulation of cell growth, proliferation, and survival pathways.
Automatically generated - may contain errors
Lab products found in correlation
Anti rsk2, by Cell Signaling Technology (2 mentions)
Polyvinylidene difluoride membrane, by Thermo Fisher Scientific (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Odyssey instrument, by LI COR (1 mentions)
Anti ube2t, by Abcam (1 mentions)
Anti p erk, by Cell Signaling Technology (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
Anti β actin, by Abcam (1 mentions)
Anti gapdh, by Abcam (1 mentions)
Anti msk1, by Cell Signaling Technology (1 mentions)
Anti bax, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by Cell Signaling Technology (1 mentions)
Anti cyclin d1, by Cell Signaling Technology (1 mentions)
Anti erk2, by Cell Signaling Technology (1 mentions)
Anti pmsk1, by Cell Signaling Technology (1 mentions)
Anti p erk1 2, by Cell Signaling Technology (1 mentions)
2 protocols using anti p rsk2
1
Protein Extraction and Western Blot Analysis
2
Protein Expression Profiling by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The extracted protein from cells was separated on 10% sodium dodecyl sulfate polyacrylamide gels and transferred to nitrocellulose membranes at 100 mA for 2 h. The membranes were blocked in skimmed milk for 1 h at room temperature and overnight at 4°C in anti-CyclinD1, anti-Bcl-2, anti-Bax (1:1,000) and anti-ERK2, anti-p-ERK1/2, anti-RSK2, anti-p-RSK2, anti-MSK1 and anti-p-MSK1 (1:800; Cell Signaling Technology, Danvers, MA, USA), respectively, before they were conjugated with the secondary antibody. Signals were observed using Cano Scan (LiDE110, Tokyo, Japan).
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