Gsta4

Manufactured by Proteintech

Sourced in United States

GSTA4 is a protein that belongs to the Glutathione S-Transferase (GST) family. GSTs are a group of enzymes that play a role in the detoxification of various compounds in the body. The primary function of GSTA4 is to catalyze the conjugation of reduced glutathione to a wide range of electrophilic and hydrophobic compounds, facilitating their metabolism and excretion.

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Lab products found in correlation

2 protocols using gsta4

Protein Expression Analysis Protocol

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Total membranes, protein and nuclear extracts were prepared and protein concentrations were determined as described previously [6 (link), 7 (link)]. The dilution of primary antibodies were as follows: CYP7B1 (1:2000), CYP8B1 (1:2000), CYP27A1 (1:2000), CYP3A4 (1:4000), UGT2B (1:4000), SULT2A1 (1:2000), GSTA1 (1:1000), GSTM2 (1:1000), OSTα (1:4000), OCT1 (1:2000), ABCG5 (1:3000), ABCG8 (1:2000), VDR (1:1000), RARα (1:1600), HNF1α (1:2000), HNF4α (1:2000), and LXR (1:1000) (Santa Cruz Biotechnology, Santa Cruz, CA), CYP2B6 (1:2000) (OriGene, Rockville, MD), GSTA2 (1:4000) (GeneTex, Irvine, CA), GSTA3 (1:1000), GSTA4 (1:1000), GSTM1 (1:1000), GSTM3 (1:1000), GSM4 (1:1000), PPARα (1:1000), and AHR (1:1000) (Proteintech Group, Chicago, IL, USA), OSTβ (1:500) (Sigma-Aldrich Chemical Co., St Louis, MO), ABCG2 (1:2000), FXR (1:10,000), SHP (1:1000), HNF3β (1:10,000), and NRF2 (1:10,000) (Abcam, Cambridge, MA). GAPDH (1:40,000) (Abcam) and SH-PTP1 (1:1600) (Santa Cruz) were used for normalizing data.

Chai J., Feng X., Zhang L., Chen S., Cheng Y., He X., Yang Y., He Y., Wang H., Wang R, & Chen W. (2015). Hepatic Expression of Detoxification Enzymes Is Decreased in Human Obstructive Cholestasis Due to Gallstone Biliary Obstruction. PLoS ONE, 10(3), e0120055.

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Western Blot Analysis of Oxidative Stress Markers

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Western blotting for SOD2 (ABCAM, Cambridge, MA), catalase (Sigma, Saint Louis, MO), Superoxide dismutase 1 (ABCAM, Cambridge, MA), Glutathione Peroxidase 1 (Gpx1) (ABCAM, Billerica, MA), Heat Shock Protein 70 (Stressgen/Enzo Lifesciences Farmingdale, NY), GSTA4 (Protein Tech, Chicago, IL), GSTπ (MBL International, Woburn, MA), GSTμ (ABCAM, Cambridge, MA), and anti-GAPDH (Millipore, Billerica, MA) was performed from 10μg of whole cell liver extracts as previously described (Shearn et al., 2014 (link); Shearn et al., 2011 (link); Shearn et al., 2013a (link); Shearn et al., 2013b (link)). Quantification of expression of each protein was performed using ImageJ (NIH) and normalized to overall GAPDH expression.

Shearn C.T., Orlicky D.J, & Petersen D.R. (2017). Dysregulation of antioxidant responses in patients diagnosed with concomitant Primary Sclerosing Cholangitis/Inflammatory Bowel Disease. Experimental and molecular pathology, 104(1), 1-8.

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