Eicosapentaenoic acid epa

T0901317 (catalog no. 2373) was purchased from Tocris Bioscience (Wiesbaden-Nordenstadt, Germany), U-18666A (catalog no. BML-S200) from Enzo Life Sciences (Lausen, Switzerland), staurosporine (catalog no. S-9300) from LC Laboratories (Woburn, MA, USA), triparanol (catalog no. T5200) and calcium ionophore A23187 (catalog no. C7522) from Merck (Darmstadt, Germany), desmosterol (catalog no. 14943), peroxide-free arachidonic acid (AA) (catalog no. 17948), eicosapentaenoic acid (EPA) (catalog no. 17949), and docosahexaenoic acid (DHA) (catalog no. 17950) from Cayman Chemical (Ann Arbor, MI, USA), and recombinant human IL-4 (catalog no. 200-04) and IL-13 (catalog no. 200-13) from PeproTech (Hamburg, Germany). Primers were purchased from Biomers GmbH (Ulm, Germany) and their sequences are available on request. All chemicals were of the highest grade of purity and commercially available.

Benzo(a)pyrene (BP), benz(a)anthracene (BA), chrysene, benzo(b)fluoranthene (BbF), benzo(k)fluoranthene (BkF), and dibenz(a,h)anthracene (DBA) were procured from Sigma-Aldrich (St. Louis, MO, USA). Indeno(1,2,3-c,d)pyrene (IP) was obtained from Absolute Standards (Hamden, CT, USA). Fish oil (from menhaden) was from Sigma-Aldrich (St. Louis, MO, USA). Omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were from Cayman Chemical (Ann Arbor, MI, USA). Materials for DNA extraction [31 (link),32 (link),33 (link),34 (link)], RNA isolation [35 (link)], and ³²postlabeling methods [31 (link),36 (link),37 (link)] have been published previously.