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Horseradish peroxidase conjugated secondary antibodies and chemiluminescence

Manufactured by GE Healthcare
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Horseradish peroxidase-conjugated secondary antibodies are enzyme-linked detection reagents commonly used in Western blotting, ELISA, and immunohistochemistry techniques. They bind to primary antibodies and catalyze a chemiluminescent reaction, producing light that can be detected and quantified.

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Lab products found in correlation

Anti p s6k1, by Cell Signaling Technology (1 mentions) Anti s6k1, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti tigar, by Abcam (1 mentions) Anti pdcd4, by Cell Signaling Technology (1 mentions) Anti p akt, by Abcam (1 mentions) Anti β actin, by Merck Group (1 mentions) Autochemi detection system, by Analytik Jena (1 mentions)

Close spelling variants of this product

Horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence Secondary horseradish peroxidase antibodies Secondary horseradish-conjugated antibodies Horseradish peroxidase

2 protocols using horseradish peroxidase conjugated secondary antibodies and chemiluminescence

1

Immunoblot Analysis of Cell Signaling Proteins

Cited 1 time
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Whole cell lysates were prepared as described [11 (link)]. Soluble proteins were analyzed by immunoblotting with anti-MUC1-C (Ab5; Thermo Scientific), anti-TIGAR (Abcam), anti-p-Akt, anti-Akt, anti-p-S6K1, anti-S6K1, anti-PDCD4 (Cell signaling Technology) and anti-β-actin (Sigma). Reactivity was detected with horseradish peroxidase-conjugated secondary antibodies and chemiluminescence (GE healthcare).
Ahmad R., Alam M., Hasegawa M., Uchida Y., Al-Obaid O., Kharbanda S, & Kufe D. (2017). Targeting MUC1-C inhibits the AKT-S6K1-elF4A pathway regulating TIGAR translation in colorectal cancer. Molecular Cancer, 16, 33.
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2

Protein Fractions Isolation and Analysis

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Preparation of total protein, cytoplasmic and nuclear fractions was described before.3 (link), 14 (link) Co-immunoprecipitation was performed as described54 (link), 56 (link) using 1 μg of TP53-antibody, FOXO3-antibody or control-IgG and 0.5% sodium-deoxycholate for effective nuclear lysis. Immunoblotting was done as described before.3 (link) Primary antibodies (Supplementary Table S1) were detected with horseradish-peroxidase-conjugated secondary antibodies and chemiluminescence (GE Healthcare, Little Chalfont, UK) and analyzed using an UVP AutoChemi-detection-system.
Rupp M., Hagenbuchner J., Rass B., Fiegl H., Kiechl-Kohlendorfer U., Obexer P, & Ausserlechner M.J. (2017). FOXO3-mediated chemo-protection in high-stage neuroblastoma depends on wild-type TP53 and SESN3. Oncogene, 36(44), 6190-6203.
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