Urea nitrogen test

Albumin, urea, LDH and TNF-α were measure as previously reported.17 The albumin ELISA kit was purchased from Bethyl Laboratories (Montgomery TX), the LDH CytoTox 96 kit from ProMega (Madison, WI), urea nitrogen test from StanBio Laboratory (Boerne, TX) and human TNF-α from ThermoFisher Scientific (Waltham, MA). Urea nitrogen, LDH and the TNF-α assays require 10 μL of media each per sample per time point. The assays were conducted following the manufacturer's guidelines with the exception of the urea nitrogen test, which was reconfigured to a 384 well format.17 ,18 The albumin ELISA measurement was determined from 1 μl of media per sample per time point following the manufacture's instructions. All media efflux assay measurements were collected with the SpectraMax M2 (Molecular Devices, Sunnyvale CA) microtiter plate reader. A Data Import Tool (DIT) was used to process raw data from biochemical assays and format the processed data for uploading into the MPS-Db. The DIT is designed to process raw data using a logistic, polynomial or linear regression algorithm to best fit the standard curve for calculating test sample results and format the processed data for uploading into the MPS-Db. For the studies reported here, the logistic regression fit was used.