Log In Sign up
The largest database of trusted experimental protocols

Cd86 e710

Manufactured by Thermo Fisher Scientific
Visit Supplier

The CD86-e710 is a cell surface marker protein produced by Thermo Fisher Scientific. It is commonly used in flow cytometry analysis to identify and characterize immune cells.

Automatically generated - may contain errors

Lab products found in correlation

Cd11c apc, by BD (2 mentions) Cd83 pe, by Thermo Fisher Scientific (2 mentions) Cd163 apc, by BioLegend (2 mentions) Cd3 apc, by Miltenyi Biotec (2 mentions) Hla dr fitc, by Miltenyi Biotec (2 mentions) Zombie dye yellow staining, by BioLegend (2 mentions) Cd45 vioblue450, by Cytek Biosciences (2 mentions) Cd3 viogreen, by Miltenyi Biotec (2 mentions) Cd103 pe cy7, by Thermo Fisher Scientific (2 mentions) Cd11c percp cy5, by BioLegend (2 mentions) Dc sign pe, by R&D Systems (2 mentions) Ccr7 pe cy7, by Miltenyi Biotec (2 mentions) Cd1a af700, by BioLegend (2 mentions) Cd1a fitc, by Thermo Fisher Scientific (2 mentions) Dc sign apc, by R&D Systems (2 mentions) Ccr5 pe cy7, by BD (2 mentions) Cd207 apc, by BioLegend (2 mentions) Dc sign fitc, by R&D Systems (2 mentions) Cd11b pe, by Cytek Biosciences (2 mentions) Cd1c pe dazzle, by BioLegend (2 mentions)

2 protocols using cd86 e710

1

Multicolor Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD45-vioblue450, CD11b-PE (Tonbo, San Diego, CA), CD11c-APC, CCR5-PE-Cy7 (BD Biosciences, San Jose, CA), CCR7-PE-Cy7, HLA-DR-FITC, CD3-VioGreen (Miltenyi Biotec), CD3-APC, CD11c-PerCp-Cy5.5, CD1c-PE-dazzle, CD163-APC, HLA-DR-BV570, CD207-APC, CD1a AF700 (Biolegend), CD103-PE-Cy7, CD83-PE, CD14-e780, CD1a-FITC, CD86-e710 (eBiosciences, San Diego, CA), DC-SIGN-FITC, DC-SIGN-PE, DC-SIGN-APC (R&D systems, Minneapolis, MN). Dead cells were excluded with 7AAD (Southern Biotech) or zombie dye yellow staining (Biolegend). Analysis was performed on 8-color MACSQuant 10 (Miltenyi biotech) or Gallios (Beckman Coulter) flow cytometers and data analyzed with FlowJo software (Tree Star, Inc. Ashland, OR). Expression of surface markers is shown as percentage of positive cells. Fluorescence minus one (FMO) strategy was used to establish appropriate gates. The gating strategy is shown in supplementary Figure 1.
Rodriguez-Garcia M., Shen Z., Barr F.D., Boesch A.W., Ackerman M.E., Kappes J.C., Ochsenbauer C, & Wira C.R. (2016). Dendritic Cells from the Human Female Reproductive Tract Rapidly Capture and respond to HIV. Mucosal immunology, 10(2), 531-544.
+ Open protocol
+ Expand
2

Multicolor Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD45-vioblue450, CD11b-PE (Tonbo, San Diego, CA), CD11c-APC, CCR5-PE-Cy7 (BD Biosciences, San Jose, CA), CCR7-PE-Cy7, HLA-DR-FITC, CD3-VioGreen (Miltenyi Biotec), CD3-APC, CD11c-PerCp-Cy5.5, CD1c-PE-dazzle, CD163-APC, HLA-DR-BV570, CD207-APC, CD1a AF700 (Biolegend), CD103-PE-Cy7, CD83-PE, CD14-e780, CD1a-FITC, CD86-e710 (eBiosciences, San Diego, CA), DC-SIGN-FITC, DC-SIGN-PE, DC-SIGN-APC (R&D systems, Minneapolis, MN). Dead cells were excluded with 7AAD (Southern Biotech) or zombie dye yellow staining (Biolegend). Analysis was performed on 8-color MACSQuant 10 (Miltenyi biotech) or Gallios (Beckman Coulter) flow cytometers and data analyzed with FlowJo software (Tree Star, Inc. Ashland, OR). Expression of surface markers is shown as percentage of positive cells. Fluorescence minus one (FMO) strategy was used to establish appropriate gates. The gating strategy is shown in supplementary Figure 1.
Rodriguez-Garcia M., Shen Z., Barr F.D., Boesch A.W., Ackerman M.E., Kappes J.C., Ochsenbauer C, & Wira C.R. (2016). Dendritic Cells from the Human Female Reproductive Tract Rapidly Capture and respond to HIV. Mucosal immunology, 10(2), 531-544.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!