Ciprofloxacin

Two tmexCD1-toprJ1 positive K. pneumoniae (RGT9-1, RGF15-2-1) and one tet(A) mutant positive K. pneumoniae RGF131 were isolated from swine faeces in 2020 by our lab. All the isolates mediate high level resistance to tigecycline (TIG) with the MIC value over than 16 µg/mL. The TIG, ciprofloxacin, meropenem, gentamicin, and metformin were purchased from Yuanye Biological Technology Company (Shanghai, China). Other fluorescence probes and reagents were purchased from Sigma-Aldrich (Saint Louis, MO, USA).

The antibiotic resistance profiles of the LAB and yeast were conducted by the method described by Nami et al. (2014 (link)). Ten antibiotics (Shanghai yuanye Bio‐Technology Co., Ltd), including ampicillin, chloramphenicol, ciprofloxacin, kanamycin, gentamicin, penicillin, tetracycline, vancomycin, clindamycin, and levofloxacin, were selected for sensitivity test of LAB strain. Identification of antibiotic susceptibility by inhibition zone diameter: susceptible >20 mm, intermediate resistant 15–19 mm, and resistant ≤14 mm (Clinical and Laboratory Standards Institute, 2012 ). Seven antibiotics including econazole, itraconazole, clotrimazole, ketoconazole, miconazole, amphotericin, and fluconazole were selected for yeast antibiotic sensitivity test. Identification of yeast antibiotic susceptibility by inhibition zone diameter: susceptible≥20 mm, intermediate resistant 10–20 mm, and resistant ≤10 mm (Clinical and Laboratory Standards Institute, 2012 ).