PFA‐fixed samples were embedded in paraffin and sectioned at 4 µm. Sections of before and after decellularization samples were stained with hematoxylin and eosin (H&E) or 4′,6‐diamidino‐2‐phenylindole (DAPI; Vectashield, Vectorlabs). H&E stained slides were imaged with Zeiss Axiokop 20 microscope and captured with a Nikon DS‐U1 camera. DAPI stained slides were analyzed using EVOS microscope (Thermo Fisher Scientific).
Immunohistochemistry (IHC) staining was performed on before and after decellularization samples with Collagen Type I and Collagen type IV (Table S4). Antigen retrieval was performed in citrate buffer (pH = 6.0) at subboiling temperatures for 10 min. Primary antibodies were incubated over night at 4°C. Envision + system horseradish peroxidase antirabbit secondary antibody (DAKO) was incubated at RT for 60 min, before staining with 3'‐diaminobenzidine and and counterstaining with hematoxylin.
Immunohistochemistry (IHC) staining was performed on before and after decellularization samples with Collagen Type I and Collagen type IV (Table S4). Antigen retrieval was performed in citrate buffer (pH = 6.0) at subboiling temperatures for 10 min. Primary antibodies were incubated over night at 4°C. Envision + system horseradish peroxidase antirabbit secondary antibody (DAKO) was incubated at RT for 60 min, before staining with 3'‐diaminobenzidine and and counterstaining with hematoxylin.