Rotor gene 3000 rt pcr system

Manufactured by Qiagen

Sourced in Japan

The Rotor-Gene 3000 RT PCR system is a real-time PCR cycler designed for quantitative gene expression analysis. It features a rotating sample carousel that can accommodate up to 72 reaction tubes. The system utilizes fluorescence detection to monitor the amplification of target DNA or RNA sequences in real-time.

Automatically generated - may contain errors

Lab products found in correlation

Sybr green kit, by Takara Bio (1 mentions) Sybr green based pcr master mix kit, by Takara Bio (1 mentions) M mlv reverse transcriptase kit, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Rotor-Gene 3000 system (45 citations) Rotor-Gene 3000 (321 citations) Rotor-Gene (167 citations)

2 protocols using rotor gene 3000 rt pcr system

Real-Time PCR Analysis of Osteogenic Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded in 24-well plates at 1 × 10⁴ cells/well and cultured for 7 days. Total RNA was isolated from cells by use of the RNA ex Pro Reagent Kit (AG, China), in accordance with the manufacturer’s instructions. cDNA was synthesized by use of the Evo M-MLV Kit (AG, China). The cDNA was then analyzed by real-time PCR analysis by use of the SYBR green kit (Takara, Japan) and Rotor-Gene 3000 RT PCR system (Corbett, Australia). Expression values were normalized to GAPDH. The primer sequences of BMP-2, osteocalcin (OCN), and PI3K are shown in Table 1.

Table 1

Polymerase chain reaction primer sequences

Target	Primer sequences (5′-3′)	Product size (bp)
GAPDH	F TTGTCTCCTGCGACTTCAACA	182
GAPDH	R GTGGTCCAGGGTTTCTTACTCC	182
BMP-2	F TAAGGATTAGCAGGTCTTTG	440
BMP-2	R CACAACCATGTCCTGATAAT	440
OCN	F AGGAGGGCAATAAGGTAGTGAA	165
OCN	R TACCATAGATGCGTTTGTAGGC	165
PI3K	F CGGTTTCTCCCTTCTACTTCCTG	83
PI3K	R GCTCTGCCTCAGCCTTTTATTG	83

GAPDH Glyceraldehyde-3-phosphate dehydrogenase, BMP-2 Bone morphogenetic protein-2, OCN Osteocalcin, PI3K Phosphoinositide 3-kinase

Dong K., Zhou W.J., Liu Z.H, & Hao P.J. (2021). The extract of concentrated growth factor enhances osteogenic activity of osteoblast through PI3K/AKT pathway and promotes bone regeneration in vivo. International Journal of Implant Dentistry, 7, 70.

+ Open protocol

+ Expand

Quantitative Real-Time PCR Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated by TRIzol reagent (Invitrogen, Carlsbad, CA, USA). RNA quantity was measured by the NanoDrop2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA) and RNA integrity was assessed by standard denaturing agarose gel electrophoresis. Complementary DNA synthesis was performed using the M-MLV reverse transcriptase kit (Invitrogen) following the manufacturer's instructions. qRT-PCR was performed using a SYBR green-based PCR master mix kit (Takara, Shiga, Japan) on a Rotor Gene 3000 RT-PCR system (Corbett Research, Sydney, Australia). The PCR condition was as follows: initial denaturation at 95°C for 30 seconds, followed by 40 cycles of 95°C for 5 seconds, and thereafter 60°C for 20 seconds. During each extension the fluorescence signal was monitored at 585 nm. GAPDH and U6 served as an internal control.

Ma L., Han C., Peng T., Li N., Zhang B., Zhen X, & Yang X. (2016). Ang-(1-7) inhibited mitochondrial fission in high-glucose-induced podocytes by upregulation of miR-30a and downregulation of Drp1 and p53. Journal of the Chinese Medical Association : JCMA, 79(11).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!