HeLa-GFP cells were seeded at 7.5 × 105 cells/mL on a 150 mm sterile dish, allowed to set for 24 h and treated with 0.25 μmol/L Doxo for additional 24 h. Populations were sorted as described above and treated for 24 h with 0.5 μmol/L Doxo. Cell population analysis was performed in a Beckman Coulter cytofluorometer.
Cytofluorometer
Manufactured by Beckman Coulter
The Cytofluorometer is a laboratory instrument used for the analysis and quantification of particles, cells, and other biological samples. It utilizes the principles of flow cytometry to measure and analyze various physical and biochemical properties of individual cells or particles as they pass through a laser beam.
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Lab products found in correlation
2 protocols using cytofluorometer
1
Doxorubicin-Induced HeLa Cell Stress
2
GFP-HeLa Cell Culture and Sorting
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HeLa-GFP cells were seeded at 7.5 × 105 cells/mL on a 150 mm sterile dish, allowed to set for 24 h. Cultures were submitted to HX for 4 days in Thermo Electron Corp. incubator. Populations were sorted in a Beckman Coulter MOFLO High Speed Cell Sorter, according to the size and GFP signal intensity as explained in the main text. After sorting, cells were seeded in 6-well plates at a cell density of 1.5 × 105 cells/mL, treated or not with 5 μmol/L SVT016426 and placed for additional 6 days in HX. Cell population analysis was performed using a Beckman Coulter cytofluorometer.
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