Px866

Manufactured by Cayman Chemical

PX866 is a selective inhibitor of the phosphoinositide 3-kinase (PI3K) enzyme. It primarily targets the PI3Kα isoform. PX866 is commonly used in scientific research applications to study the role of the PI3K signaling pathway.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using px866

Cell Culture Media and Supplements

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture media and supplements such as RPMI 1640, DMEM, PBS, and antibiotics (penicillin, streptomycin, and puromycin) were purchased from Lonza (Allendale, NJ), Fisher Scientific (Pittsburgh, PA) or Santa Cruz Biotechnology (Dallas, TX). Heat-inactivated fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Flowery Branch, GA). Sodium butyrate and sanguinarine were procured from Sigma-Aldrich (St. Louis, MO). Trichostatin A (TSA), apicidin, HC toxin, LY294002, PX866, CAL-101, MK2206, Triciribine, GDC0068, Rapamycin, AZD8055, and BEZ235 were obtained from Cayman Chemical (Ann Arbor, MI). Tetrandrine was acquired from Santa Cruz Biotechnology, and (–)-depudecin was purchased from BioVision (Milpitas, CA) and MyBioSource (San Diego, CA). Datiscetin was ordered from BOC Sciences (Shirley, NY), while wortmannin and CUDC-907 were procured from Selleck Chemicals (Houston, TX).

Lyu W., Deng Z., Sunkara L.T., Becker S., Robinson K., Matts R, & Zhang G. (2018). High Throughput Screening for Natural Host Defense Peptide-Inducing Compounds as Novel Alternatives to Antibiotics. Frontiers in Cellular and Infection Microbiology, 8, 191.

+ Open protocol

+ Expand

Antibody Validation for AKT/GLUT4 Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies against Sort1 (ab16640; Lot: GR64653-1), GLUT4 (ab48547; Lot: Ab654) and α-Tubulin (ab7291; Lot: GR122217-1) were from Abcam (Cambridge, MA). Antibodies against phospho-AKT (Ser-473; 4060; Lot: 19), AKT (4691; Lot: 17), ubiquitin (3933), LC3B (3868, Lot: 9), and Histone 3 (9717; Lot: 8), TNFα and wortmannin were purchased from Cell Signaling Technology (Danvers, MA). Antibody against GGA2 (H-175, sc-30103; Lot: K0305) was purchased from Santa Cruz Biotechnology (Dallas, Texas). Antibodies against FLAG (M2) (F1804; Lot: SLBJ4607V) and Actin (A5441; Lot: 063M4808), Anti-FLAG (M2) magnetic beads, AKT1/2 inhibitor VIII, cycloheximide, chloroquine, Bafilomycin A1, MG132, and TBCA were purchased from Sigma (St. Louis, MO). The antibody against phospho-Sort1 (S825) was developed by Epitomics (Burlingame, CA). Insulin was purchased from Eli Lilly and Company (Indianapolis, IN). PX866 was purchased from Cayman Chemical (Ann Arbor, MI). Alexa Fluor 488 IgG (A-21202; Lot: 1572559) and Alexa Fluor 594 (A-21207; Lot: 1558726) were purchased from Life Technologies (Grand Island, NY).

Li J., Chen C., Li Y., Matye D.J., Wang Y., Ding W.X, & Li T. (2017). Inhibition of insulin/PI3K/AKT signaling decreases adipose Sortilin 1 in mice and 3T3-L1 adipocytes. Biochimica et biophysica acta, 1863(11), 2924-2933.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!