The largest database of trusted experimental protocols

3 protocols using 1 2 aminoethyl piperazine

1

Synthesis of Fluorescent Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sodium citrate (cat# 71402), EDCI (cat# E7750), sodium N-hydroxysulfosuccinimide (cat# 56485), AgNO3 (cat# 209139), perylene-3,4,9,10-tetracarboxylic dianhydride (PTCDA) (cat# P11255), L-ascorbic acid (cat# A92902) benzylhexadecyldimethylammonium chloride (BDAC) (cat# B4136), hexadecyltrimethylammonium bromide (CTAB) (cat# 52365), 1-(2-aminoethyl)piperazine (cat# A55209), and an Amicon ultra centrifugal filter (50 kDa, MWCO) (cat# UFC9050) were purchased from Sigma Aldrich (St. Louis, MO, USA). HAuCl4 × H2O (cat# 12325) and HSPEG3000COOH (cat# PEG1099) were purchased from Alfa Aesar (Waltham, MA, USA) and IRIS Biotech (Marktredwitz, Germany), respectively.
+ Open protocol
+ Expand
2

Charge-Reducing Reagents for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Trimethylamine N-oxide was purchased from Cayman Chemical. Spermidine was purchased from Beantown Chemical. Spermine was purchased from Alfa Aesar. Histamine, bis(2-aminoethyl)amine, bis(3-aminopropyl)amine, tris(2-aminoethyl)amine, 3,3′-diamino-N-methyldipropylamine, tris-(3-aminopropyl)amine, piperazine, 1-(2-aminoethyl)-piperazine, and 1,4-bis(3-aminopropyl)piperazine were purchased from Sigma-Aldrich. Protein was buffer exchanged into 200 mM ammonium acetate (supplemented with 0.5% C8E4 for membrane protein). Charge-reducing reagents stocks were made by dissolving into the same buffer as the protein and were mixed with protein to obtain desired final concentrations of charge-reducing reagent.
+ Open protocol
+ Expand
3

Polycationic Hydrogel Fabrication and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Poly(allylamine hydrochloride) (Mw=120,000-200,000) was purchased from Thermo Fisher Scientific, Sweden and was purified and modified prior to use. Potassium hydroxide (pellets, 85%-100%), polyvinylalcohol (Mw=89,000-98,000, 89% hydrolyzed), 1-Ethyl-3-(3dimethylaminopropyl) carbodiimide, N-hydroxysulfosuccinimide, 2-bromopropane, Methanol (anhydrous, >99.8), 4arm-poly(ethylene glycol)-NH2 (Mn=5,000), 8arm-poly(ethylene glycol)-NH2 (hexaglycerol core, Mn=10,000), Trimethylolpropane tris[poly(propylene glycol), amine terminated] ether (Mw=1368), β-Alanine (99%), 1-(2-Aminoethyl) piperazine (99%) and Sarcosine (98%) were used as received from Sigma-Aldrich, Norway. Microfibrillated cellulose (MFC, originated from eucalyptus pulp) and sodium carboxymethylated cellulose (CMC, Na + content 2830 µmol/g) were provided by INOFIB, France, as suspensions in water with a solid content of 3.3 and 1.7 wt%, respectively.
Polyvinylidene fluoride (PVDF) Ultrafiltration membrane (50k MW) with polypropylene (PP) substrate was provided by Synder Filtration, USA. 3M™ Fluorinert™ Electronic Liquid FC-72 was used as received from Kemi-Intressen, Sweden. The gases used in the gas permeation tests included a CO2/N2 mixture (10 vol.% CO2 in N2) and CH4 (99.95%) were purchased from AGA, Norway and used as received.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!