Cd44 buv737

Manufactured by BD

The CD44 BUV737 is a fluorophore-conjugated antibody that binds to the CD44 cell surface glycoprotein. It is designed for use in flow cytometry applications to identify and analyze cells expressing CD44.

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2 protocols using cd44 buv737

Comprehensive Immunophenotyping Protocol

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Antibodies for flow cytometry and FACS: H2Kb PECy7 (eBioscience 25-5958-82), H2Db PE (BioLegend 111508), I-A/I-E (MHCII) BV711 (BD Biosciences 563414), PDL1 BV41 (BD Biosciences 564716), CD8 APC-eFluor 780 (eBioscience 47-0081-82), DAPI (Sigma, D9564-10 MG), PD1 PerCP-eFluor710 (Invitrogen 46-9985-82), CD44 BUV737 (BD Biosciences 564392), CD62 L BV786 (BD Biosciences 564109), hTCRab PerCP-Cy5.5 (Biolegend 306724), hCD3 PECy7 (BD Biosciences 563423). p15E (KSPWFTTL), OVA (SIINFEKL) peptides and corresponding pentamers were from ProImmune.

Ye X., Waite J.C., Dhanik A., Gupta N., Zhong M., Adler C., Malahias E., Ni M., Wei Y., Gurer C., Zhang W., Macdonald L.E., Murphy A.J., Sleeman M.A, & Skokos D. (2020). Endogenous retroviral proteins provide an immunodominant but not requisite antigen in a murine immunotherapy tumor model. Oncoimmunology, 9(1), 1758602.

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Multiparametric Flow Cytometry for Immune Profiling

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First, single cell suspensions were incubated for 20 min in Fixable Viability Dye (FVD) eFluor™ 780 (eBioscience) in the dark on ice to exclude dead cells. After adding 1 µg TruStain™ FcX (Biolegend), immunostaining was performed on ice for 30 min (protected from light) with the following antibodies: CD45-AF700 (# 103,128), CD45-BV785 (# 103,149), Ly6C-APC (# 128,015), Ly6C-FITC (# 128,005), Ly6G-PE/Dazzle594 (# 127,648), CD11c-BV605 (# 117,334), CD8-PerCPCy5.5 (# 100,734), PD1-APC (# 135,210), CD62L-BV605 (# 104,438), CD44-FITC (# 103,005), CD19-FITC (# 115,506), CD19-PECy7 (# 115,520), PD1-BV421 (# 135,221) (all antibodies from Biolegend), and CD11b-BUV737 (# 612,801), F4/80-PE (# 565,410), Siglec-F-PErCP5.5 (# 565,526), F4/80-BUV395 (# 565,614), CD3-BUV395 (# 563,565), CD4-BUV496 (# 564,667), CD44-BUV737 (# 612,799), Siglec-F-PE (# 562,068) (all antibodies from BD Biosciences).
After staining, cells were washed and fixed using IC Fixation Buffer (eBioscience). Samples were either analyzed on a BD LSRFortessa™ or BD Canto II™ flow cytometer with FACSDiva software (BD Biosciences). Analyses and compensation were performed with Flowjo software (TreeStar). Fluorescence minus-one-samples were used to define gates. See Kienzl et al. [10] (link) (Supplementary material) for gating strategies.

Gruden E., Kienzl M., Hasenoehrl C., Sarsembayeva A., Ristic D., Schmid S.T., Maitz K., Taschler U., Hahnefeld L., Gurke R., Thomas D., Kargl J, & Schicho R. (2023). Tumor microenvironment-derived monoacylglycerol lipase provokes tumor-specific immune responses and lipid profiles. Prostaglandins, leukotrienes, and essential fatty acids, 196.

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