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Ibitreat μ slide chemotaxis chambers

Manufactured by Ibidi
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Sourced in Germany

The IbiTreat μ-slide chemotaxis chambers are lab equipment designed for cell migration studies. They provide a controlled environment for observing and analyzing cellular responses to chemical gradients.

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Lab products found in correlation

Bovine collagen type 1, by Merck Group (2 mentions) Observer z1, by Zeiss (2 mentions)

Spelling variants of this product

μ-slide chemotaxis chamber (16 citations) IbiTreat μ-Slide (16 citations) μ-slide chambers (12 citations) IbiTreat chamber slides (10 citations) μ-Slide Chemotaxis (9 citations) IbiTreat chambers (9 citations) µ-Slide Chemotaxis chambers (7 citations) Chemotaxis μ-slides (5 citations) μ-Chamber slides (4 citations) μ-Slide Chemotaxis ibiTreat (3 citations)

2 protocols using ibitreat μ slide chemotaxis chambers

1

Chemotaxis Assay of Immune Cells

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BMDCs, BMDMs or human macrophages were challenged as indicated, washed, resuspended in CM with 1 mg/mL bovine collagen type I (Sigma) and seeded into gelatin (1%)-coated or uncoated ibiTreat μ-slide chemotaxis chambers (Ibidi, Martinsried, Germany). Collagen was allowed to polymerize for 45 minutes and media, inhibitors and 1,25 μg/mL murine (BMDCs, BMDMs) or human recombinant CCL19 (human macrophages) were added as indicated conform the manufacturer’s instructions (application note 23). Cells were then imaged every 2,5 (BMDCs) or 5 (BMDMs, human macrophages) min for 4h (BMDCs) or 8h (BMDMs, human macrophages; Zeiss Observer Z.1). Motility tracks for 35–60 cells per condition were analyzed using ImageJ software for each experiment.
ten Hoeve A.L., Braun L., Rodriguez M.E., Olivera G.C., Bougdour A., Belmudes L., Couté Y., Saeij J.P., Hakimi M.A, & Barragan A. (2022). The Toxoplasma effector GRA28 promotes parasite dissemination by inducing dendritic cell-like migratory properties in infected macrophages. Cell host & microbe, 30(11), 1570-1588.e7.
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2

Macrophage Motility Tracking by Chemotaxis

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BMDMs or human macrophages were challenged as indicated, washed, resuspended in CM with 1 mg/mL bovine collagen type I (Sigma) and seeded into uncoated ibiTreat μ-slide chemotaxis chambers (Ibidi, Martinsried, Germany). Collagen was allowed to polymerize for 30 min and media, inhibitors and 1,25 μg/mL murine (BMDMs) or human recombinant CCL19 (human macrophages) were added as indicated conform the manufacturer’s instructions (application note 23). Cells were then imaged every 5 min for 8h (Zeiss Observer Z.1). Motility tracks for ≥35 cells per condition were analyzed using ImageJ software for each experiment.
ten Hoeve A.L., Rodriguez M.E., Säflund M., Michel V., Magimel L., Ripoll A., Yu T., Hakimi M.A., Saeij J.P., Ozata D.M, & Barragan A. (2024). Hypermigration of macrophages through the concerted action of GRA effectors on NF-κB/p38 signaling and host chromatin accessibility potentiates Toxoplasma dissemination. bioRxiv.
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