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Human endothelial basal growth medium

Manufactured by Thermo Fisher Scientific
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Human endothelial basal growth medium is a cell culture media formulation designed to support the growth and maintenance of human endothelial cells in vitro. It provides the necessary nutrients and growth factors required for the proliferation and survival of these specialized cells.

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Lab products found in correlation

Percoll, by Cytiva (4 mentions) Dynabead, by Thermo Fisher Scientific (4 mentions) Hepatocyte growth factor (hgf), by Thermo Fisher Scientific (3 mentions) Collagenase type 1a, by Merck Group (2 mentions) Collagenase ia, by Merck Group (2 mentions) Vascular endothelial growth factor (vegf), by Thermo Fisher Scientific (2 mentions) Rat tail collagen, by Merck Group (1 mentions)

Close spelling variants of this product

Endothelial basal medium Endothelial growth medium Human Endothelial Medium Medium basal

4 protocols using human endothelial basal growth medium

1

Isolation of Liver Endothelial Cells

Cited 1 time
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Tissue samples and blood samples from patients were obtained with written informed consent and with local ethics committee approval (LREC reference 06/Q2702/61, Birmingham, UK and 04/Q2708/41, Birmingham, UK). Liver endothelial cells were isolated from explanted livers or donor tissue surplus to surgical requirements using a collagenase digestion (collagenase type 1a, Sigma-Aldrich) as described previously60 (link). All tissue was collected from patients in the Liver Unit at Queen Elizabeth Hospital in Birmingham with informed consent and under local ethics committee approval. Briefly, digested tissue was placed over a 33% / 77% Percoll (Amersham Biosciences) density gradient. The endothelial cells were isolated by immunomagnetic selection using Abs against CD31 conjugated to Dynabeads (Life Technologies). The endothelial cells were then cultured in medium composed of human endothelial basal growth medium (Life technologies), 10% AB human serum (HD supplies), 10ng/ml vascular endothelial growth factor (VEGF), and 10ng/ml hepatocyte growth factor (HGF) (Peprotech). The cells were grown in collagen-coated culture flasks and were maintained at 37°C in a humidified incubator with 5% CO2 until confluent.
Hoare M., Ito Y., Kang T.W., Weekes M.P., Matheson N.J., Patten D.A., Shetty S., Parry A.J., Menon S., Salama R., Antrobus R., Tomimatsu K., Howat W., Lehner P.J., Zender L, & Narita M. (2016). NOTCH1 mediates a switch between two distinct secretomes during senescence. Nature cell biology, 18(9), 979-992.
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2

Isolation of Liver Endothelial Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue samples and blood samples from patients were obtained with written informed consent and with local ethics committee approval (LREC reference 06/Q2702/61, Birmingham, UK and 04/Q2708/41, Birmingham, UK). Liver endothelial cells were isolated from explanted livers or donor tissue surplus to surgical requirements using a collagenase digestion (collagenase type 1a, Sigma-Aldrich) as described previously60 (link). All tissue was collected from patients in the Liver Unit at Queen Elizabeth Hospital in Birmingham with informed consent and under local ethics committee approval. Briefly, digested tissue was placed over a 33% / 77% Percoll (Amersham Biosciences) density gradient. The endothelial cells were isolated by immunomagnetic selection using Abs against CD31 conjugated to Dynabeads (Life Technologies). The endothelial cells were then cultured in medium composed of human endothelial basal growth medium (Life technologies), 10% AB human serum (HD supplies), 10ng/ml vascular endothelial growth factor (VEGF), and 10ng/ml hepatocyte growth factor (HGF) (Peprotech). The cells were grown in collagen-coated culture flasks and were maintained at 37°C in a humidified incubator with 5% CO2 until confluent.
Hoare M., Ito Y., Kang T.W., Weekes M.P., Matheson N.J., Patten D.A., Shetty S., Parry A.J., Menon S., Salama R., Antrobus R., Tomimatsu K., Howat W., Lehner P.J., Zender L, & Narita M. (2016). NOTCH1 mediates a switch between two distinct secretomes during senescence. Nature cell biology, 18(9), 979-992.
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3

Isolation and Culture of Human Liver Endothelial Cells

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HSECs were isolated from approximately 30 g human liver tissue as described previously.14 Briefly, tissue was subjected to collagenase digestion (10 mg/mL collagenase IA; Sigma‐Aldrich, Gillingham, Dorset, UK) and was placed on a 33%/77% Percoll (Amersham Biosciences, Little Chalfont, Buckinghamsire, UK) density gradient. The nonparenchymal cell layer was then removed, and the endothelial cells were isolated by positive immunomagnetic selection utilizing CD31 antibody‐conjugated Dynabeads (Thermo Fisher, Bishop Meadow Road, Loughborough, UK). The endothelial cells were then cultured in medium composed of human endothelial basal growth medium (Thermo Fisher) supplemented with 10% human serum (HD Supplies, Botolph Claydon, Buckinghamshire, UK), 10 ng/mL vascular endothelial growth factor (PeproTech, London, UK), and 10 ng/mL hepatocyte growth factor (PeproTech). The cells were grown in rat tail culture vessels coated with collagen (1 in 100; Sigma‐Aldrich) and were maintained at 37°C in a humidified incubator with 5% CO2. Human umbilical vein endothelial cells (HUVECs) isolated using standard methods14 were used as a control endothelial cell line.
Patten D.A., Wilson G.K., Bailey D., Shaw R.K., Jalkanen S., Salmi M., Rot A., Weston C.J., Adams D.H, & Shetty S. (2016). Human liver sinusoidal endothelial cells promote intracellular crawling of lymphocytes during recruitment: A new step in migration. Hepatology (Baltimore, Md.), 65(1), 294-309.
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4

Isolation and Culture of Human Liver Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HSEC were isolated from ~30 g human liver tissue as described previously(14 (link)). Briefly, tissue was subjected to collagenase digestion (10 mg/ml collagenase IA; Sigma-Aldrich) and was placed on a 33 %/77 % Percoll (Amersham Biosciences) density gradient. The non-parenchymal cell layer was then removed, and the endothelial cells were isolated by positive immunomagnetic selection utilising CD31 antibody-conjugated Dynabeads (Thermo Fisher). The endothelial cells were then cultured in medium composed of human endothelial basal growth medium (Thermo Fisher) supplemented with 10 % human serum (HD Supplies), 10 ng/ml vascular endothelial growth factor (VEGF; PeproTech), and 10 ng/ml hepatocyte growth factor (HGF; PeproTech). The cells were grown in rat tail collagen (1 in 100; Sigma-Aldrich)-coated culture vessels and were maintained at 37°C in a humidified incubator with 5 % CO2. HUVEC, isolated using standard methods(14 (link)), were used as a control endothelial cell line.
Patten D.A., Wilson G.K., Bailey D., Shaw R.K., Jalkanen S., Salmi M., Rot A., Weston C.J., Adams D.H, & Shetty S. (2016). Human liver sinusoidal endothelial cells promote intracellular crawling of lymphocytes during recruitment: A new step in migration. Hepatology (Baltimore, Md.), 65(1), 294-309.
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