Caspase 3 colorimetric assay kit casp 3 c

Manufactured by Merck Group

Sourced in United Kingdom

The Caspase-3 colorimetric assay kit (CASP-3-C) is a laboratory tool designed to detect and measure the activity of caspase-3, a key enzyme involved in the process of apoptosis or programmed cell death. The kit provides a simple and reliable method for quantifying caspase-3 activity in cell lysates or purified samples.

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Sigma’s Caspase-3 Colorimetric Assay Kit (CASP-3-C, Caspase-3 colorimetric assay kit Caspase 3 colorimetric assay CASP-3-C Caspase colorimetric assay kit Caspase-3 assay kit Colorimetric assay kit Colorimetric assay Caspase-3

2 protocols using caspase 3 colorimetric assay kit casp 3 c

Silica Nanoparticle Synthesis and Characterization

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Triton X-100, cyclohexane (≥99.8%), tetraethyl orthosilicate (TEOS ≥99.0%), ammonium hydroxide (NH₄OH, 28.0–30.0%), aminopropyltriethoxysilane (APTES), ethanol absolute (≥99.8%), nitric acid (HNO₃ > 90%), hydrochloric acid (HCl 37%), Dulbecco's Modified Eagle's Medium-high glucose (DMEM), Leibovitz (L15) medium, fetal bovine serum (FBS), penicillin-streptomycin, dimethyl sulfoxide (DMSO), WST-8 assay, phosphate buffer saline (PBS), silicon standard, 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI), trypsin, glutaraldehyde, FITC-phalloidin, and caspase-3 colorimetric assay kit (CASP-3-C) were purchased from Sigma Aldrich, Dorset, UK. Petri dishes and multiwell were purchased from Corning® Costar®. All the reagents were used as received without further purification.

De Matteis V., Cascione M., De Luca A., Manno D.E, & Rinaldi R. (2022). High Doses of Silica Nanoparticles Obtained by Microemulsion and Green Routes Compromise Human Alveolar Cells Morphology and Stiffness Differently. Bioinorganic Chemistry and Applications, 2022, 2343167.

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Caspase-3 Activity Measurement

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Caspase-3 activity was measured in the supernatant 24 and 48 h after irradiation with the Caspase-3 Colorimetric Assay Kit (CASP-3-C, Sigma, St. Louis, MO) according to the manufacturer's instructions.

Perona M., Thomasz L., Rossich L., Rodriguez C., Pisarev M.A., Rosemblit C., Cremaschi G.A., Dagrosa M.A, & Juvenal G.J. (2018). Radiosensitivity enhancement of human thyroid carcinoma cells by the inhibitors of histone deacetylase sodium butyrate and valproic acid. Molecular and cellular endocrinology, 478.

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