A total of 60 GBM patients were analyzed for the presence of CD20+ B cells by IHC. Formalin-fixed paraffin-embedded tumor blocks of GBM patients (see Table 1 for patients’ characteristics) were double-labeled with rabbit anti-human CD20 (1:2 dilution, clone EP459Y, Thermo Fischer) and mouse anti-human CD8 (1:100 dilution, clone 4B11, Leica). Anti-rabbit and anti-mouse HRP secondary antibodies (both from Abcam) were used at 1:500 dilution. Twenty consecutive high-power fields were evaluated on each tumor by a board-certified neuropathologist from the Neurosurgical Department at Northwestern University. Images were captured on a Leica DMi8 inverted microscope.
Anti rabbit and anti mouse hrp secondary antibodies
Manufactured by Abcam
Anti-rabbit and anti-mouse HRP secondary antibodies are detection reagents used in various immunoassays and immunoblotting techniques. They are conjugated with the enzyme horseradish peroxidase (HRP) and are designed to bind to primary antibodies raised in rabbit or mouse. The HRP enzyme can be used to generate a colorimetric or chemiluminescent signal, enabling the visualization and detection of target proteins or analytes.
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Lab products found in correlation
2 protocols using anti rabbit and anti mouse hrp secondary antibodies
1
Quantifying GBM B Cell Infiltration
2
Quantifying Tumor-Infiltrating Lymphocytes in GBM
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Formalin-fixed paraffin-embedded tumor blocks of 60 GBM patients were double-labeled with rabbit anti-human CD20 (1:2 dilution, clone EP459Y, Thermo Fischer) and mouse anti-human CD8 (1:100 dilution, clone 4B11, Leica). Anti-rabbit and anti-mouse HRP secondary antibodies (both from Abcam) were used at 1:500 dilution. Twenty consecutive high-power fields were evaluated on each tumor by a board-certified neuropathologist from the Neurosurgical Department at Northwestern University. Images were captured on a Leica DMi8 inverted microscope.
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