Gfp del

Manufactured by Vienna Drosophila Resource Center

The GFP-Del is a laboratory equipment used for visualizing specific cellular structures or processes in Drosophila (fruit flies). It utilizes green fluorescent protein (GFP) technology to label and track targeted proteins or organelles within the flies. The core function of the GFP-Del is to enable researchers to observe and study the dynamics and localization of proteins of interest in live Drosophila samples.

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2 protocols using gfp del

Drosophila Transgenic Stocks for ChIP-seq

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The following stocks were used: bam^Δ86 (BDSC5427), bam^Df (BDSC27403), C(1)RM (BDSC9460), nos-Gal4 (BDSC4937), UASp-shRhi (BDSC35171), iso-1 (BDSC2057), GFP-Cid (BDSC25047), GFP-D1 (BDSC50850) were obtained from Bloomington Drosophila Stock Center; GFP-Rhi (VDRC313340), GFP-Del (VDRC313271), GFP-Cuff (VDRC313269), moon^Δ1 (VDRC313735), moon^Δ28 (VDRC313738), Bam-GFP (VDRC318001), w¹¹¹⁸ (VDRC60000) were obtained from Vienna Drosophila Resource Center; tj-Gal4 (DGRC104055) was obtained from Kyoto Stock Center; rhi², rhi^KG and UASp-GFP-Rhi were gifts of William Theurkauf; del^WK36, del^HN56, cuff^QQ37and cuff^WM25 were gifts of Trudi Schüpbach; GFP-Vasa (gift of Paul Lasko), copiaLTR-lacZ (gift of Elena Pasyukova), GFP-Moon (gift of Peter Andersen), Sa-GFP (gift of Xin Chen). UASp-mKate2 was described before [3 (link)]. GFP-tagged Rhi, Del, Cuff and Moon are previously described transgenes constructed by inserting N-terminal GFP into genomic BACs via recombineering [2 (link),8 (link)]. XO males were generated by crossing GFP-Rhi males to C(1)RM females. To perform GFP-Rhi ChIP, bam^Δ86 and GFP-Rhi were recombined.

Chen P., Luo Y, & Aravin A.A. (2021). RDC complex executes a dynamic piRNA program during Drosophila spermatogenesis to safeguard male fertility. PLoS Genetics, 17(9), e1009591.

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Drosophila Germline Stem Cell Genetics

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The following stocks were used: bam ∆86 (BDSC5427), bam Df (BDSC27403), C(1)RM (BDSC9460), nos-Gal4 (BDSC4937), UASp-shRhi (BDSC35171), iso-1 (BDSC2057) were obtained from Bloomington Drosophila Stock Center; GFP-Rhi (VDRC313340), GFP-Del (VDRC313271), GFP-Cuff (VDRC313269), moon ∆1 (VDRC313735), moon ∆28 (VDRC313738), Bam-GFP (VDRC318001) were obtained from Vienna Drosophila Resource Center; tj-Gal4 (DGRC104055) was obtained from Kyoto Stock Center; rhi 2 , rhi KG and UASp-GFP-Rhi were gifts of William Theurkauf; del WK36 , del HN56 , cuff QQ37 and cuff WM25 were gifts of Trudi Schüpbach; GFP-Vasa (gift of Paul Lasko), copiaLTR-lacZ (gift of Elena Pasyukova), GFP-Moon (gift of Peter Andersen), Sa-GFP (gift of Xin Chen). UASp-mKate2 was described before (Chen et al., 2016) (link). XO males were generated by GFP-Rhi males crossed to C(1)RM females. bam ∆86 and GFP-Rhi were recombined for testis ChIP.

Chen P., Luo Y., & Aravin A.A. (2020). RDC complex executes a dynamic piRNA program duringDrosophilaspermatogenesis to safeguard male fertility.

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