Anti fbw7 a301 720a

Chemicals were purchased from the following manufacturers: cycloheximide (CHX), doxycycline (Doxy), sodium butyrate (NaB), MG132, and etoposide were obtained from Sigma; A-1210477 was obtained from Cayman Chemical; and AT-101 was procured from Apex Bio. Primary antibodies were purchased from the following manufacturers: anti-MCL-1 (4572), anti-ubiquitin (3933), anti-mTOR (7959), and anti-KSHV LANA (LNA-1; 4013) were purchased from Abcam; anti-FBW7 (A301-720A) was obtained from Bethyl; anti-MCL-1 (sc-12756), anti-GST (sc-138), anti-Lamin A/C (sc-625), anti-ORF45 (sc-53883), and anti-K8.1 (sc-65446) were obtained from Santa Cruz; anti-β-actin (A-5441), anti-FLAG (F1804), and anti-tubulin (GTU-88) were purchased from Sigma; anti-V5 (MA5-15253) was obtained from Invitrogen; anti-Au (901901) was purchased from BioLegend; anti-HA (M180-3) was procured from MBL; anti-Cyclin E (2983) and anti-COXIV (4850) were purchased from cell signaling technology; anti-K2 (13-214-050) was obtained from ABI. Myc antibody was kindly provided by Dr. Jin-Hyun Ahn (Sungkyunkwan University). Anti-RTA and anti-ORF6 antibodies were generously provided by Dr. Yoshihiro Izumiya (University of California, Davis) and Dr. Gary Hayward (Johns Hopkins University), respectively.

The anti-ABL1 (2862, 1:1000), anti-p27 (3686, 1:1000), anti-DEK (13962, 1:1000), anti-SRC3 (2126, 1:1000), anti-CUL3 (2759, 1:1000), anti-GST (2625, 1:2000), anti-β-TRCP (4394, 1:1000), anti-p-ERK(9101, 1:1000), and anti-ERK (4695, 1:1000) antibodies were obtained from Cell Signaling Technology. Anti-ERG (EPR3864, 1:1000) antibody was obtained from Abcam. Anti-SPOP (16750-1-AP, 1:1000) antibody was obtained from Proteintech. Anti-GFP (A-11122, 1:5000) antibody was obtained from Thermo Fisher. Anti-FBW7 (A301-720A, 1:1000) and anti-PML (A301-167A, 1:1000) were obtained from Bethyl Laboratories. Anti-CCDC6 (sc-100309, 1:1000) and anti-α Tubulin (sc-8035, 1:2000) antibodies were obtained from Santa Cruz Biotechnoloy. Mouse monoclonal anti-HA.11 epitope tag (clone 16B12, 901513, 1:1000) was obtained from BioLegend. Anti-Vinculin (V9131, 1:50000), rabbit polyclonal anti-HA (H6908, 1:3000), mouse monoclonal ANTI-FLAG® M2 (F3165, 1:5000), rabbit polyclonal ANTI-FLAG® (F7425, 1:3000), anti-mouse IgG (whole molecule)-peroxidase (A4416, 1:5000), and anti-rabbit IgG (whole molecule)-peroxidase (A4914, 1:5000) were obtained from Sigma-Aldrich. Mouse monoclonal ANTI-FLAG® M2 affinity agarose gel (A2220) and mouse monoclonal anti-HA-agarose (A2095) were obtained from Sigma-Aldrich.