Hesc qualified matrigel coated plates

Manufactured by BD

HESC-qualified Matrigel-coated plates are laboratory equipment designed for cell culture applications. They provide a pre-coated surface that supports the growth and attachment of various cell types. The Matrigel coating is a complex mixture of extracellular matrix proteins that mimics the natural cellular environment.

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Lab products found in correlation

Knockout serum replacer, by Thermo Fisher Scientific (1 mentions) Human recombinant bfgf, by Thermo Fisher Scientific (1 mentions) Mitomycin c, by Merck Group (1 mentions) Mtesr1 medium, by STEMCELL (1 mentions) Gentle cell dissociation buffer, by STEMCELL (1 mentions) Stemmacs ips brew xf medium, by Miltenyi Biotec (1 mentions)

2 protocols using hesc qualified matrigel coated plates

Maintenance of Pluripotent Stem Cells

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hESC line H9 and hIPSCs were maintained on MEFs, mitotically inactivated by mitomycin C (Sigma Aldrich), and DMEM/F-12 medium supplemented with 20% Knockout serum replacer, 0.1 mM nonessential amino acids, 1 mM L-glutamine, 0.1 mM β mercaptoethanol, 1× penicillin-streptomycin, and 10 ng/mL human recombinant bFGF (all from Invitrogen). After 7 days of culture, cells were manually plucked for glutaraldehyde fixation.
For feeder-free experiments, pluripotent stem cells were cultured on hESC-qualified Matrigel-coated plates (BD Biosciences) in mTeSR1 medium (Stem Cell Technologies) according to manufacturer's guidelines. Colonies were manually harvested as for feeder dependent cultures.

Courtot A.M., Magniez A., Oudrhiri N., Féraud O., Bacci J., Gobbo E., Proust S., Turhan A.G, & Bennaceur-Griscelli A. (2014). Morphological Analysis of Human Induced Pluripotent Stem Cells During Induced Differentiation and Reverse Programming. BioResearch Open Access, 3(5), 206-216.

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Cardiac Differentiation of hiPS Cells

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The previously characterized foreskin fibroblast-derived human induced pluripotent stem (hiPS) cell clone C2a was used [24] . The hiPS cell line was maintained on mitoticallyinactivated MEFs in DMEM/F12 medium supplemented with 2 mM L-glutamine, 0.07% βmercaptoethanol, 20% knockout serum replacement, 5 ng/ml bFGF and 1% NEAA under low oxygen atmosphere (4% O 2 ). Cells were passaged by manual dissection of cell clusters every 6 to 7 days. Before cardiac differentiation, hiPS cells were manually transferred from MEFs to hESC-qualified matrigel-coated plates (0.05 mg/ml, BD Biosciences) and cultured in StemMACS (iPS-Brew XF) medium (Miltenyi Biotec). Passages were performed using Gentle Cell Dissociation Buffer (StemCell Technologies).

Es-Salah-Lamoureux Z., Jouni M., Malak O.A., Belbachir N., Al Sayed Z.R., Gandon-Renard M., Lamirault G., Gauthier C., Baró I., Charpentier F., Zibara K., Lemarchand P., Beaumelle B., Gaborit N, & Loussouarn G. (2016). HIV-Tat induces a decrease in I_Kr and I_Ksvia reduction in phosphatidylinositol-(4,5)-bisphosphate availability. Journal of molecular and cellular cardiology, 99.

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